In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Fluorescein-12-dUTP could be used in directly enzymatic labeling of DNA/cDNA, such as PCR, random primed DNA labeling reaction, primer extension, Nick Translation and others. It can be incorporated as a substitute for its natural counterpart dTTP. The Dye-labeled DNA/cDNA probes acquired are ideally suited for fluorescence hybridization applications - FISH or microarray-based gene expression profiling. Optimal substrate properties and labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine. Recommendatory fluorescein-12-dUTP/dTTP ratio for PCR and Nick Translation: 30-50% Fluorescein-12-dUTP/ 70-50% dTTP. Please note: Please protect the Dye-labeled dUTP from exposure to light and experiment under low light conditions. The optimal final concentration of the Dye-labeled dUTP might very depending on the practical application and assay conditions. For generating optimal product yields and high incorporation rates, an individual optimization for the Dye-labeled-dUTP/dTTP ratio is recommended.
Fluorescein-12-dUTP is used as a substrate for:
- Taq polymerase
- phi29 DNA Polymerase
- DNA Polymerase I (holoenzyme and Klenow fragment)
- Terminal Transferase
- Reverse transcriptase (e.g., from AMV and M-MuLV).
. Wiegant et al. (2001) Probe Labeling and Fluorescence in situ Hybridization. Current Protocols in Cytometry 8:8.3.1.
. Yie SM1, Xiao R, et, al. Progesterone regulates HLA-G gene expression through a novel progesterone response element. Hum Reprod. 2006 Oct;21(10):2538-44. Epub 2006 May 9.
|Storage||store at -20°C|
|M.Wt||994.69 (free acid)|
|Formula||C39H41N4O21P3 (free acid)|
|Synonyms||Fluorescein-X-5-Aminoallyl-dUTP; 5/6-Fluorescein-X-5-aminoallyl-2'-deoxyuridine-5'-triphosphate, Triethylammonium salt|
|Solubility||Soluble in sterile water，pH 7.5 ±0.5|
|Canonical SMILES||OC1=CC(O2)=C(C3(C4=C2C=C(O)C=C4)C5=C(C(O3)=O)C=C(C(NCCCCCC(NC/C([H])=C([H])/C(C(N6)=O)=CN(C6=O)[[email protected]@]7([H])C[[email protected]@](O)([H])[[email protected]@](O7)([H])COP(O)(OP(O)(OP(O)(O)=O)=O)=O)=O)=O)C=C5)C=C1|
|Shipping Condition||Evaluation sample solution: ship with blue ice. All other available sizes: ship with RT, or blue ice upon request.|
|General tips||For obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. Stock solution can be stored below -20°C for several months.|