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Fluorescein-12-dUTP

Catalog No.
B8207
Incorporated into DNA/cDNA by PCR, Nick Translation or other application for dye-labeled probe producing, used in FISH, microarray and other aspects
Grouped product items
SizePriceStock Qty
25ul (1mM)
$200.00
In stock
5x25ul (1mM)
$840.00
In stock

Tel: +1-832-696-8203

Email: [email protected]

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Background

Fluorescein-12-dUTP could be used in directly enzymatic labeling of DNA/cDNA, such as PCR, random primed DNA labeling reaction, primer extension, Nick Translation and others. It can be incorporated as a substitute for its natural counterpart dTTP. The Dye-labeled DNA/cDNA probes acquired are ideally suited for fluorescence hybridization applications - FISH or microarray-based gene expression profiling. Optimal substrate properties and labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine. Recommendatory fluorescein-12-dUTP/dTTP ratio for PCR and Nick Translation: 30-50% Fluorescein-12-dUTP/ 70-50% dTTP. Please note: Please protect the Dye-labeled dUTP from exposure to light and experiment under low light conditions. The optimal final concentration of the Dye-labeled dUTP might very depending on the practical application and assay conditions. For generating optimal product yields and high incorporation rates, an individual optimization for the Dye-labeled-dUTP/dTTP ratio is recommended.

Fluorescein-12-dUTP is used as a substrate for:

- Taq polymerase

- phi29 DNA Polymerase

- DNA Polymerase I (holoenzyme and Klenow fragment)

- Terminal Transferase

- Reverse transcriptase (e.g., from AMV and M-MuLV).

References:
[1]. Wiegant et al. (2001) Probe Labeling and Fluorescence in situ Hybridization. Current Protocols in Cytometry 8:8.3.1.
[2]. Yie SM1, Xiao R, et, al. Progesterone regulates HLA-G gene expression through a novel progesterone response element. Hum Reprod. 2006 Oct;21(10):2538-44. Epub 2006 May 9.

Chemical Properties

Physical AppearanceSolution
Storagestore at -20°C
M.Wt994.69 (free acid)
Cas No.214154-36-6
FormulaC39H41N4O21P3 (free acid)
SynonymsFluorescein-X-5-Aminoallyl-dUTP; 5/6-Fluorescein-X-5-aminoallyl-2'-deoxyuridine-5'-triphosphate, Triethylammonium salt
SolubilitySoluble in sterile water,pH 7.5 ±0.5
SDFDownload SDF
Canonical SMILESOC1=CC(O2)=C(C3(C4=C2C=C(O)C=C4)C5=C(C(O3)=O)C=C(C(NCCCCCC(NC/C([H])=C([H])/C(C(N6)=O)=CN(C6=O)[[email protected]@]7([H])C[[email protected]@](O)([H])[[email protected]@](O7)([H])COP(O)(OP(O)(OP(O)(O)=O)=O)=O)=O)=O)C=C5)C=C1
Shipping ConditionEvaluation sample solution: ship with blue ice. All other available sizes: ship with RT, or blue ice upon request.
General tipsFor obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. Stock solution can be stored below -20°C for several months.

Quality Control

Quality Control & MSDS

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Chemical structure

Fluorescein-12-dUTP