In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
FLLL32 is a small molecule analog of curcumin. It can inhibit signal transducer and activator of transcription-3 (STAT3). In the pSTAT3-positive A375 cell line, FLLL32 resulted in pro-apoptotic effects with an IC50 value of 1.3 μM at 48 hours .
Activation of STAT3 in melanoma tumors is related to poor prognosis. STAT3 can promote angiogenesis and cell proliferation, drive invasion and metastasis, and inhibit apoptosis .
In multiple human melanoma cell lines, after a 24 hour treatment with FLLL32, the STAT3 phosphorylation at Tyr705 but not at Tyr727 was inhibited. In a prior in vitro cell-free assay, FLLL32 was found to inhibit the activity of Jak2 kinase. But in the experiment with multiple human melanoma cell line, no appreciable alteration in the phosphorylation of Jak2, even when the concentration of FLLL32 was 8 μM, was observed. This meant that FLLL32 likely directly acted against the STAT3 protein. FLLL32 at concentrations ranged from 2-4 μM for only 4 hours reduced pSTAT3 and induce cell death .
In an s.c. model of B16F10 melanoma in C57BL/6 mice, compared to the treatment with DMSO to the same animals, daily i.p. administration of FLLL-32 at concentrations of 25 mg/kg and 50 mg/kg significantly delayed tumor growth (p<0.001) .
. Bill MA, Fuchs JR, Li C, et al. The small molecule curcumin analog FLLL32 induces apoptosis in melanoma cells via STAT3 inhibition and retains the cellular response to cytokines with anti-tumor activity. Molecular cancer, 2010, 9(1): 165.
. Lesinski G, Bill M, Li C, et al. Abstract# 856: The small molecule curcumin analog FLLL-32 induces apoptosis in human melanoma cells via STAT3 inhibition and elicits in vivo anti-tumor activity. Cancer Research, 2009, 69(9 Supplement): 856-856.
|Physical Appearance||A crystalline solid|
|Storage||Store at -20°C|
|Solubility||insoluble in H2O; ≥2.58 mg/mL in EtOH; ≥55.4 mg/mL in DMSO|
|Shipping Condition||Evaluation sample solution: ship with blue ice. All other available sizes: ship with RT, or blue ice upon request.|
|General tips||For obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. Stock solution can be stored below -20°C for several months.|