HyperScript™ First-Strand cDNA Synthesis SuperMix
- 1. Yeping Chen, Rongyuan Liang, et al. "Identification of ZNF652 as a Diagnostic and Therapeutic Target in Osteoarthritis Using Machine Learning." Journal of Inflammation Research Volume 17, 2025 - Issue
- 2. Zequn Liu, Yanmin Jiang, et al. "ASXL3 gene mutations inhibit cell proliferation and promote cell apoptosis in mouse cardiomyocytes by upregulating lncRNA NONMMUT063967.2." Biochem Biophys Rep. 2023 Jun 27:35:101505. PMID: 37435360
- 3. Yunjie Wei, Junhao Zhao, et al. "Wogonin reduces cardiomyocyte apoptosis from mitochondrial release of cytochrome cto improve doxorubicin‑induced cardiotoxicity." Exp Ther Med. 2022 Mar;23(3):205. PMID: 35126708
- 4. Yongqiang Zhou, Jinjing Hu, et al. "Gly-tRF enhances LCSC-like properties and promotes HCC cells migration by targeting NDFIP2." Cancer Cell Int. 2021 Sep 18;21(1):502. PMID: 34537070
HyperScript™ First-Strand cDNA Synthesis SuperMix is based on HyperScript™ Reverse Transcriptase (Cat. No. K1071) to synthesize first-strand cDNA from Total RNA or Poly(A)+ RNA. HyperScript™ Reverse Transcriptase is a new enzyme obtained through genetic engineering based on M-MLV (RNase H-) Reverse Transcriptase. In comparison, HyperScript™ Reverse Transcriptase reduces RNase H activity and increases thermal stability. HyperScript™ Reverse Transcriptase can withstand higher reaction temperatures and is suitable for reverse transcription of RNA templates with complex secondary structures. In addition, HyperScript™ Reverse Transcriptase enhances the affinity to the template, suitable for the reverse transcription of a small number of templates and low copy genes, and can generate cDNA up to 12.3 kb.
HyperScript™ First-Strand cDNA Synthesis SuperMix contains all components except primers required for the synthesis of the first strand cDNA and provides two cDNA synthesis primers, Random Primers and Oligo (dT)23VN. You can choose Random Primers, Oligo (dT)23VN or gene-specific primers as reverse transcription primers according to the needs of the experiment. The synthesized first-strand cDNA products can be used in subsequent experiments such as PCR amplification and qPCR reaction.
| Components | 50 rxns (20 μL reaction) | 100 rxns (20 μL reaction) |
| RNase Free ddH2O | 1 mL | 1 mL x 2 |
| 5X SuperMix | 200 μL | 400 μL |
| Random Primers (50 μM) | 50 μL | 100 μL |
| Oligo (dT) 23VN (50 μM) | 50 μL | 100 μL |
| 5X control Mix | 20 μL | 40 μL |
Store the components at -20°C. | ||
SuperMix contains all components except primers required for the synthesis of the first strand cDNA, easy to use.
Efficient HyperScript™ Reverse Transcriptase for RNA templates containing secondary structures
Synthesis of first-strand cDNA up to 12.3 kb
Detects template starting amounts as low as 1 pg
Oligo (dT)23VN primers have stronger template anchoring ability and higher reverse transcription efficiency than Oligo (dT)18
Synthesized first-strand cDNA can be used for experiments such as PCR amplification and qPCR reaction
