EZ Cap™ Reagent GG
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
EZ Cap Reagent GG is a co-transcriptional capping reagent for in vitro transcription of 5’ capped mRNA resulting in a Cap 1 structure. EZ Cap GG requires a GG initiator. EZ Cap GG typically provides >90% capped material generating a natural Cap 1 structure.
EZ Cap is compatible with wild-type T7 polymerase. 5 mM Cap and rNTPs is recommended.
Template design is an integral part of any transcription. EZ Cap Reagent GG should be used with a specific initiating sequence as 5’ GG 3’.
Final capping efficiency is dependent on the EZ Cap Reagent, DNA template and final mRNA sequence. Secondary structure due to RNA length and base composition may have an influence on final capping efficiency.
Unlike Cap AG, using Cap GG might lead to the comparable ARCA transcriptional stuttering and yield.
|Storage||Store at -20°C or below|
|M.Wt||1161.6 (free acid)|
|Formula||C33H45N15O24P4 (free acid)|
|Synonyms||EZ Cap™ Reagent GG for co-transcriptional capping of mRNA, m7G(5')ppp(5')(2'OMeG)pG|
|Shipping Condition||Evaluation sample solution: ship with blue ice. All other available sizes: ship with RT, or blue ice upon request.|
|General tips||For obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. Stock solution can be stored below -20°C for several months.|