|EZ Cap™ Reagent GG
Used for co-transcriptional capping of mRNA to form Cap 1 structure, m7G(5')ppp(5')(2'OMeG)pG
Sample solution is provided at 25 µL, 10mM.
Publications citing ApexBio Products
|Cas No.||SDF||Download SDF|
|Synonyms||CleanCap™ Reagent GG for co-transcriptional capping of mRNA, m7G(5')ppp(5')(2'OMeG)pG|
|Formula||C33H45N15O24P4 (free acid)||M.Wt||1161.6 g/mol (free acid)|
|Solubility||Storage||Store at -20°C or below|
|Shipping Condition||Evaluation sample solution : ship with blue ice.All other available size: ship with RT , or blue ice upon request|
|General tips||For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.|
EZ Cap Reagent GG is a co-transcriptional capping reagent for in vitro transcription of 5’ capped mRNA resulting in a Cap 1 structure. EZ Cap GG requires a GG initiator. EZ Cap GG typically provides >90% capped material generating a natural Cap 1 structure.
EZ Cap is compatible with wild-type T7 polymerase. 5 mM Cap and rNTPs is recommended.
Template design is an integral part of any transcription. EZ Cap Reagent GG should be used with a specific initiating sequence as 5’ GG 3’.
Final capping efficiency is dependent on the EZ Cap Reagent, DNA template and final mRNA sequence. Secondary structure due to RNA length and base composition may have an influence on final capping efficiency.
Unlike Cap AG, using Cap GG might lead to the comparable ARCA transcriptional stuttering and yield.