EZ Cap™ Reagent GG

Size	Price	Stock
10 µl (100 mM)	$222.00	Please Inquire
50 µl (100 mM)	$1,026.00	Please Inquire

Publications citing ApexBio Products

Nature.
2017 Jan 19;541(7637):417-420.

Nature.
2018 Jun 13.

Nature.
2018 Jun 27.

Nature.
2018 Mar 29;555(7698):673-677.

Nature.
2017 Sep 7;549(7670):96-100.

Nature.
2016 Apr 21;532(7599):398-401.

Science.
2016 Aug 5;353(6299)594-8

Nat Nanotechnol.
2017 Dec;12(12):1190-1198.

Nature Biotechnology.
2017 Jun;35(6):569-576

Cell.
2018 Jun 28;174(1):172-186.e21.

Cell.
2018 Feb 22;172(5):1007-1021.e17.

Cell.
2017 Nov 30;171(6):1284-1300.e21.

Cell.
2017 Aug 17. pii: S0092-8674(17)30869-3.

Cell.
2017 Jul 13;170(2):312-323

Nat Med.
2018 Jan 29.

Nat Med.
2017 Nov;23(11):1342-1351.

Cell.
2017 Apr 6;169(2):286-300.

Cell.
2015 Aug 27;162(5):987-1002.

Cell.
2015 Feb 12;160(4):729-44.

Nature Medicine.
2017 Apr;23(4):493-500.

Cancer Cell.
2018 May 14;33(5):905-921.e5.

Cancer Cell.
2018 Apr 9;33(4):752-769.e8.

Cancer Cell.
2018 Mar 12;33(3):401-416.e8.

Cancer Cell.
2017 Aug 14;32(2):253-267.e5.

Nat Methods.
2018 Jul;15(7):523-526.

Cell Stem Cell.
2018 May 3;22(5):769-778.e4.

Cell Stem Cell.
2017 Nov 20. pii: S1934-5909(17)30375-2.

Cancer Cell.
2017 May 8;31(5):635-652.e6.

Immunity.
2018 Jan 16;48(1):59-74.e5.

Immunity.
2017 Oct 17;47(4):635-647.e6.

Immunity.
2016 Jan 19;44(1):88-102

Nat Cell Biol.
2018 Feb;20(2):186-197.

Quality Control

Quality Control & MSDS

View current batch:

Purity = ≥95% by AX-HPLC

COA (Certificate Of Analysis)
MSDS (Material Safety Data Sheet)

Chemical structure

EZ Cap™ Reagent GG Dilution Calculator

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EZ Cap™ Reagent GG Molarity Calculator

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Chemical Properties

Cas No.	N/A	SDF	Download SDF
Synonyms	CleanCap™ Reagent GG for co-transcriptional capping of mRNA, m7G(5')ppp(5')(2'OMeG)pG
Chemical Name	N/A
Canonical SMILES	O=C1NC(N)=NC2=C1N=CN2C3C(O)C(O)C(COP([O-])(OC4C(OC)C(N5C(N=C(N)NC6=O)=C6N=C5)OC4COP([O-])(OP([O-])(OP([O-])(OCC7OC(N8C=[N+](C)C9=C8N=C(N)NC9=O)C(O)C7O)=O)=O)=O)=O)O3.[NH4+].[NH4+].[NH4+]
Formula	C₃₃H₄₅N₁₅O₂₄P₄ (free acid)	M.Wt	1161.6 g/mol (free acid)
Solubility	N/A	Storage	Store at -20°C or below
Shipping Condition	Evaluation sample solution : ship with blue ice.All other available size: ship with RT , or blue ice upon request
General tips	For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.

Background

EZ Cap Reagent GG is a co-transcriptional capping reagent for in vitro transcription of 5’ capped mRNA resulting in a Cap 1 structure. EZ Cap GG requires a GG initiator. EZ Cap GG typically provides >90% capped material generating a natural Cap 1 structure.

EZ Cap is compatible with wild-type T7 polymerase. 5 mM Cap and rNTPs is recommended.

Template design is an integral part of any transcription. EZ Cap Reagent GG should be used with a specific initiating sequence as 5’ GG 3’.

Final capping efficiency is dependent on the EZ Cap Reagent, DNA template and final mRNA sequence. Secondary structure due to RNA length and base composition may have an influence on final capping efficiency.

Unlike Cap AG, using Cap GG might lead to the comparable ARCA transcriptional stuttering and yield.