EZ Cap™ CBEmax mRNA (m1Ψ) 5x1mg(1mg/mL)

CBEmax is an optimized Cytosine Base Editor (CBE) tool, which can efficiently convert cytosine (C) in DNA sequences directly into thymine (T) without generating DNA double-strand breaks. By introducing CBEmax into cells in the form of mRNA, transient and efficient gene editing expression can be achieved, reducing off-target effects and the risk of genomic instability. It can be widely applied in basic research, disease model construction and gene therapy fields, especially suitable for the precise repair of genetic diseases and functional genomics research.

EZ Cap™ CBEmax mRNA (m1Ψ) is provided at a concentration of ~1 mg/ml with Cap1 structure. It expressed cytosine base editing enzyme. There are currently two ways to cap mRNA: One is co-transcription method, by adding Cap analogues into the transcription process. The other is enzymatic Capping. After transcription, Cap0 capping is performed by Vaccinia virus Capping Enzyme (VCE), GTP and S-adenosylmethionine (SAM). The Cap0 is then generated into the Cap1 through 2´-O-Methyltransferase and SAM. Cap1 Capping can also be performed by adding VCE, 2´-O-Methyltransferase, GTP and SAM in a one-step process. Cap 1 structure is more ideal for mammalian systems and possess higher transcription efficiency than Cap 0 structure. The addition of N1-Methylpseudo-UTP(m1Ψ) and poly(A) tail suppress RNA-mediated innate immune activation and increase the stability and lifetime of the mRNA in vitro and in vivo. Poly(A) tail also plays an important role in enhancing the efficiency of translation initiation.