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ddhCTP

Catalog No.
B8293
Chain terminator for the RNA-dependent RNA polymerases
Grouped product items
SizePriceStock Qty
10ul (100 mM)
$650.00
In stock
50ul (100 mM)
$2,150.00
In stock
100ul (100 mM)
$3,575.00
In stock
For scientific research use only and should not be used for diagnostic or medical purposes.

Tel: +1-832-696-8203

Email: [email protected]

Worldwide Distributors

Background

ddhCTP is a biologically occurring nucleotide analog generated by the interferon-induced enzyme Viperin. Viperin catalyzes an S-adenosyl-l-methionine (SAM)-dependent radical-mediated reaction converting cytidine triphosphate (CTP) into 3'-deoxy-3',4'-didehydro-CTP (ddhCTP). ddhCTP acts as a chain terminator targeting RNA-dependent RNA polymerases of flaviviruses, interrupting viral RNA synthesis. This molecule inhibits replication of viruses such as dengue, West Nile, and Zika viruses, demonstrating antiviral potential in mammalian cellular contexts and in vivo models, thus representing an important research tool in antiviral studies.

Chemical Properties

Physical AppearanceSolution
StorageStore at -20°C or below
M.Wt465.14
FormulaC9H14N3O13P3
Synonyms3ʹ-deoxy-3′,4ʹ-didehydro-CTP
SolubilitySoluble in H2O
Chemical Name(5-(4-amino-2-oxopyrimidin-1(2H)-yl)-4-hydroxy-4,5-dihydrofuran-2-yl)methyl tetrahydrogen triphosphate
SDFDownload SDF
Canonical SMILESOC1C=C(COP(OP(OP(O)(O)=O)(O)=O)(O)=O)OC1N2C(N=C(N)C=C2)=O
Shipping ConditionSmall Molecules with Blue Ice, Modified Nucleotides with Dry Ice.
General tips We do not recommend long-term storage for the solution, please use it up soon.

Protocol

Cell experiment [1]:

Cell lines

Mammalian HEK293T cells.

Preparation method

This compound can be soluble in H2O. General tips for obtaining a higher concentration: Please warm the tube at 37 °C for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below -20°C for several months.

Reacting condition

0~72 h, 0, 1, 10, 100 and 300 μM

Applications

ddhCTP inhibits replication of flavivirus (dengue virus, West Nile Virus and Zika virus) RNA-dependent RNA polymerase in HEK293T cells.

References:

[1] Anthony S. Gizzi, Tyler L. Grove, Jamie J. Arnold, Joyce Jose, Rohit K. Jangra, Scott J. Garforth, Quan Du, Sean M. Cahill, Natalya G. Dulyaninova, James D. Love, Kartik Chandran, Anne R. Bresnick, Craig E. Cameron, Steven C. Almo. A naturally occurring antiviral ribonucleotide encoded by the human genome. Nature, 2018; DOI: 10.1038/s41586-018-0238-4.

Quality Control

Chemical structure

ddhCTP

Related Biological Data

ddhCTP

Related Biological Data

ddhCTP