ddhCTP
ddhCTP is a biologically occurring nucleotide analog generated by the interferon-induced enzyme Viperin. Viperin catalyzes an S-adenosyl-l-methionine (SAM)-dependent radical-mediated reaction converting cytidine triphosphate (CTP) into 3'-deoxy-3',4'-didehydro-CTP (ddhCTP). ddhCTP acts as a chain terminator targeting RNA-dependent RNA polymerases of flaviviruses, interrupting viral RNA synthesis. This molecule inhibits replication of viruses such as dengue, West Nile, and Zika viruses, demonstrating antiviral potential in mammalian cellular contexts and in vivo models, thus representing an important research tool in antiviral studies.
| Physical Appearance | Solution |
| Storage | Store at -20°C or below |
| M.Wt | 465.14 |
| Formula | C9H14N3O13P3 |
| Synonyms | 3ʹ-deoxy-3′,4ʹ-didehydro-CTP |
| Solubility | Soluble in H2O |
| Chemical Name | (5-(4-amino-2-oxopyrimidin-1(2H)-yl)-4-hydroxy-4,5-dihydrofuran-2-yl)methyl tetrahydrogen triphosphate |
| SDF | Download SDF |
| Canonical SMILES | OC1C=C(COP(OP(OP(O)(O)=O)(O)=O)(O)=O)OC1N2C(N=C(N)C=C2)=O |
| Shipping Condition | Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice. |
| General tips | We do not recommend long-term storage for the solution, please use it up soon. |
| Cell experiment [1]: | |
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Cell lines |
Mammalian HEK293T cells. |
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Preparation method |
This compound can be soluble in H2O. General tips for obtaining a higher concentration: Please warm the tube at 37 °C for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below -20°C for several months. |
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Reacting condition |
0~72 h, 0, 1, 10, 100 and 300 μM |
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Applications |
ddhCTP inhibits replication of flavivirus (dengue virus, West Nile Virus and Zika virus) RNA-dependent RNA polymerase in HEK293T cells. |
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References: [1] Anthony S. Gizzi, Tyler L. Grove, Jamie J. Arnold, Joyce Jose, Rohit K. Jangra, Scott J. Garforth, Quan Du, Sean M. Cahill, Natalya G. Dulyaninova, James D. Love, Kartik Chandran, Anne R. Bresnick, Craig E. Cameron, Steven C. Almo. A naturally occurring antiviral ribonucleotide encoded by the human genome. Nature, 2018; DOI: 10.1038/s41586-018-0238-4. |
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Quality Control & MSDS
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Chemical structure
Related Biological Data
Related Biological Data
