Colorimetric Succinate Dehydrogenase Activity Assay Kit
Succinate dehydrogenase (SDH) is a cytochrome oxidase located on the inner mitochondrial membrane that provides electrons for the respiratory chain. SDH catalyzes the oxidation of succinic acid to fumarate while transferring electrons to coenzyme Q, which provides energy to the cell through the tricarboxylic acid cycle and electron transport chain. Therefore, SDH activity can be used as an indicator to evaluate the tricarboxylic acid cycle. In addition, SDH mutations may lead to many diseases, such as Ritchie syndrome. Detection of SDH activity is of great clinical significance.
The Colorimetric Succinate Dehydrogenase Activity Assay Kit is used for the rapid detection of SDH activity by colorimetric method. SDH dehydrogenates succinic acid to produce fumarate, and the removed hydrogen is delivered via PMS and reduces DCIP. DCIP is a blue substance with an absorption peak at 600 nm. The degree of reduction of DCIP can be judged by detecting changes in absorbance, which indirectly reflects the activity of SDH. SDH activity is inversely proportional to the amount of residual DCIP.
This product is rapid, simple and highly sensitive. It has a good linearity in the range of 0-100 nmol.
Figure 1: Typical SDH standard curve and assay data. (A) DCIP standard curve, (B) the kinetic mode of HeLa cells
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Components |
100 Assays |
Storage |
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Lysis Buffer |
25 mL |
-20°C |
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SDH Assay Buffer |
20 mL |
-20°C |
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DCIP (50 mM) |
200 μL |
-20°C away from light |
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SDH Substrate |
120 μL |
-20°C |
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SDH Probe |
100 μL |
-20°C away from light |
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Shipping: Blue ice Shelf life: 1 year |
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