In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Clorgyline (hydrochloride) is an irreversible, potent and selective monoamine oxidase (MAO) A inhibitor with Ki values of 0.054 and 58 μM and IC50 values of 0.0012 and 1.9 μM for MAO-A and MAO-B, respectively .
Monoamine oxidases (MAOs) are FAD depending enzymes and exists in two forms, namely MAO-A and MAO-B. MAOs are responsible for the regulation and metabolism of major monoamine neurotransmitters such as 5-hydroxytryptamine (5-HT), norepinephrine (NE), and dopamine (DA). MAO-A preferentially oxidizes serotonin. Human MAO-A inhibitors are antidepressants and antianxiety agents .
Clorgyline is an irreversible, potent and selective MAO-A inhibitor. In wild-type mice, clorgyline significantly inhibited MAO-A enzymatic activity by approximately 80%. Clorgyline also significantly elevated striatal levels of 5-HT and NE, and significantly decreased DOPAC levels, suggesting that inhibition of MAO-A activity decreased the metabolism of DA. In YAC128 HD mice, clorgyline (1.5 mg/kg for 21 days) inhibited MAO-A enzymatic activity in cortical tissue by approximately 90% and significantly elevated striatal 5-HT and NE levels. Clorgyline treatment also improved anxiety-like phenotypes and reduced depressive-like behavior .
. Abdelhafez OM, Amin KM, Ali HI, et al. Synthesis of new 7-oxycoumarin derivatives as potent and selective monoamine oxidase A inhibitors. J Med Chem. 2012 Dec 13;55(23):10424-36.
. Mazouz F, Gueddari S, Burstein C, et al. 5-[4-(benzyloxy)phenyl]-1,3,4-oxadiazol-2(3H)-one derivatives and related analogues: new reversible, highly potent, and selective monamine oxidase type B inhibitors. J Med Chem. 1993 Apr 30;36(9):1157-67.
. Garcia-Miralles M, Ooi J, Ferrari Bardile C, et al. Treatment with the MAO-A inhibitor clorgyline elevates monoamine neurotransmitter levels and improves affective phenotypes in a mouse model of Huntington disease. Exp Neurol. 2016 Apr;278:4-10.
|Storage||Store at -20°C|
|Solubility||≥17.65mg/mL in DMSO|
|Chemical Name||N-[3-(2,4-dichlorophenoxy)propyl]-N-methyl-2-propyn-1-amine, monohydrochloride|
|Shipping Condition||Evaluation sample solution : ship with blue ice.All other available size: ship with RT , or blue ice upon request|
|General tips||For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.|