In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Chidamide is a novel benzamide-type histone deacetylase (HDAC) inhibitor. People have investigated the effects of CS055 on proliferation, differentiation and apoptosis in human leukaemia cell lines and primary myeloid leukaemia cells.
Histone deacetylases (HDACs) is a series of enzymes functioning to acetylate and deacetylate the amino-terminal lysine residues of histones, which result in the remodeling of the chromatin structures and affect the accessibility of the chromatin to transcription factors to start gene transcription.
Chidamide has effect on cell cycle, it significantly reduced the S phase cell fraction, while inducing a marked increase in the G1 phase cell fraction in BEL-7402 and HCC-9204 cells with different p53 statuses. Chidamide signifiantly altered the number of cells in the phase fractions with an increase in dose.
The results of the present study suggest that Chidamide is a HDACi with potential therapeutic value in several haematological malignancies via the inhibition of cell proliferation, inducing differentiation and apoptosis in human cells. Chidamide is a new HDACi with potential therapeutic values in several haematological malignancies via the inhibition of cell proliferation, inducing differentiation and apoptosis in human leukaemia cells.
 Gong K, Xie J, Yi H, Li W. CS055 (Chidamide/HBI-8000), a novel histone deacetylase inhibitor, induces G1 arrest, ROS-dependent apoptosisand differentiation in human leukaemia cells. Biochem J. 2012 May 1;443(3):735-46.
 Wang H1, Guo Y, Fu M, Liang X, etal. , Antitumor activity of Chidamide in hepatocellular carcinoma cell lines. Mol Med Rep. 2012 Jun;5(6):1503-8.
|Physical Appearance||A solid|
|Storage||Store at -20°C|
|Solubility||insoluble in H2O; insoluble in EtOH; ≥18.55 mg/mL in DMSO|
|Chemical Name||(1Z,2E)-N-(4-((2-amino-5-fluorophenyl)carbamoyl)benzyl)-3-(pyridin-3-yl)acrylimidic acid|
|Shipping Condition||Evaluation sample solution: ship with blue ice. All other available sizes: ship with RT, or blue ice upon request.|
|General tips||For obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. Stock solution can be stored below -20°C for several months.|