CFDA-SE
CFDA-SE (i.e. carboxyfluorescein diacetate succinimidyl ester) is a stable, cell-permeable dye, composed of a fluorescein molecule containing two acetate moieties and a succinimidyl ester functional group. After diffusion into the intracellular environment, cellular esterases remove the acetyl moieties from CFDA-SE, leaving behind CFSE (i.e. carboxyfluorescein succinimidyl ester) which covalently binds to amino groups on intracellular macromolecules by its succinimidyl group and can be retained within the cells for at least 8 weeks. CFSE stained cells can then be detected by fluorescence microscopy and flow cytometry under the excitation/emission maxima of 491/518 nm. CFDA-SE provides a valuable tool to quantify cell division and track cell migration, due to the sequential decrease in fluorescent labeling in daughter cells.
References:
1. Wang XQ, Duan XM, Liu LH, et al. Carboxyfluorescein diacetate succinimidyl ester fluorescent dye for cell labeling. Acta Biochimica et Biophysica Sinica (Shanghai), 2005, 37(6): 379-385.
2. Weston SA, Parish CR. New fluorescent dyes for lymphocyte migration studies. Analysis by flow cytometry and fluorescence microscopy. Journal of Immunological Methods, 1990, 133(1): 87-97.
3. Parish CR, Glidden MH, Quah BJ, et al. Use of the intracellular fluorescent dye CFSE to monitor lymphocyte migration and proliferation. Current Protocols in Immunology, 2009, Chapter 4: Unit4.9.
4. Graziano M, St-Pierre Y, Beauchemin C, et al. The fate of thymocytes labeled in vivo with CFSE. Experimental Cell Research, 1998, 240(1): 75-85.
| Storage | Store at -20°C |
| M.Wt | 557.46 |
| Cas No. | 150347-59-4 |
| Formula | C29H19NO11 |
| Solubility | ≥37.17 mg/mL in DMSO with ultrasonic; insoluble in EtOH; insoluble in H2O |
| Chemical Name | 3',6'-bis(acetyloxy)-3-oxo-2,5-dioxo-1-pyrrolidinyl ester-spiro[isobenzofuran-1(3H),9'-[9H]xanthene]-ar-carboxylic acid |
| SDF | Download SDF |
| Canonical SMILES | CC(OC1=CC2=C(C3(C4=C(C=C(OC(C)=O)C=C4)O2)C5=C(C(O3)=O)C=C(C(ON6C(CCC6=O)=O)=O)C=C5)C=C1)=O.CC(OC7=CC8=C(C9(C%10=C(C(O9)=O)C=CC(C(ON%11C(CCC%11=O)=O)=O)=C%10)C%12=C(C=C(OC(C)=O)C=C%12)O8)C=C7)=O |
| Shipping Condition | Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice. |
| General tips | We do not recommend long-term storage for the solution, please use it up soon. |
| Cell experiment:[1] | |
|
Cell lines |
Human erythroleukaemic cell line K562, mouse lymphoma cell line YAC-1, human mammary cancer cell line MCF-7 and human melanoma cell line A375 |
|
Reaction Conditions |
1, 1.5, 2, 2.5, 5 and 10 µM CFDA-SE |
|
Applications |
CFDA-SE at 2.5 μM stained more than 95% of the cells on all cell lines tested, and dose-dependently increased fluorescence intensity of stained cells. The optimal concentration for K562 and YAC-1 was found to be 2.5 μM, while the optimal concentrations for A375 and MCF-7 were found to be 5 μM and 10 μM, respectively. Within the 6 h experiment period, no cytotoxicity related to CFDA-SE was observed. |
| Animal experiment:[4] | |
|
Animal models |
C57BL/6 mice, aged 5 ~ 8 weeks |
|
Dosage form |
10 μM Injected into thymic lobe |
|
Applications |
CFDA-SE, at 80 times the concentration used for in vitro labeling, was nontoxic and labeled randomly approximately 15% of thymocytes 24 h after injection. The turnover rate of labeled thymic emigrants in the lymph nodes was in the order of 21 days. Thus, CFDA-SE may serve as a powerful tool in relatively long-term migration studies. |
|
Note |
The technical data provided above is for reference only. |
|
References: 1. Wang XQ, Duan XM, Liu LH, et al. Carboxyfluorescein diacetate succinimidyl ester fluorescent dye for cell labeling. Acta Biochimica et Biophysica Sinica (Shanghai), 2005, 37(6): 379-385. 2. Weston SA, Parish CR. New fluorescent dyes for lymphocyte migration studies. Analysis by flow cytometry and fluorescence microscopy. Journal of Immunological Methods, 1990, 133(1): 87-97. 3. Parish CR, Glidden MH, Quah BJ, et al. Use of the intracellular fluorescent dye CFSE to monitor lymphocyte migration and proliferation. Current Protocols in Immunology, 2009, Chapter 4: Unit4.9. 4. Graziano M, St-Pierre Y, Beauchemin C, et al. The fate of thymocytes labeled in vivo with CFSE. Experimental Cell Research, 1998, 240(1): 75-85. |
|
Quality Control & MSDS
- View current batch:
Chemical structure
