Cerulenin
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Cerulenin is a widely used natural inhibitor of fatty acid synthase (FAS).
In primary rat aortic endothelial cells, the inhibitor of fatty acid synthase and HMG-CoA reductase, Cerulenin (5 μg/mL) and Lovastatin, are used before palmitic acid added. The mRNA expression of IL-1β, TNFα, VCAM-1 and IL-6 are reduced while NO production is recovered with inhibitor treatment[2].
In ob/ob mice, cerulenin effectively depletes fat content in the steatotic liver, which trigger a series of reactions: Decreased expression of PPARα and γ reduced UCP2 levels, increase liver ATP content, and reduce ROS production. Elevated intracellular ATP levels and reduced ROS ultimately improved liver function.
References:
[1]. Bitencourt T A, et al. Trans-chalcone and quercetin down-regulate fatty acid synthase gene expression and reduce ergosterol content in the human pathogenic dermatophyte Trichophyton rubrum. BMC Complement Altern Med, 2013, 13(1): 229.
[2]. Tian D, et al. Overexpression of steroidogenic acute regulatory protein in rat aortic endothelial cells attenuates palmitic acid-induced inflammation and reduction in nitric oxide bioavailability. Cardiovasc Diabetol, 2012, 11(1): 144.
[3]. Cheng G, et al. Cerulenin blockade of fatty acid synthase reverses hepatic steatosis in ob/ob mice. PLoS One, 2013, 8(9): e75980.
| Physical Appearance | A crystalline solid |
| Storage | Store at -20°C |
| M.Wt | 223.3 |
| Cas No. | 17397-89-6 |
| Formula | C12H17NO3 |
| Solubility | ≤20mg/ml in ethanol;25mg/ml in DMSO;30mg/ml in dimethyl formamide |
| Chemical Name | 2R,3S-epoxy-4-oxo-7,10-dodecadienamide |
| SDF | Download SDF |
| Canonical SMILES | C/C=C/C/C=C/CCC([[email protected]@H]1[[email protected]](C(N)=O)O1)=O |
| Shipping Condition | Evaluation sample solution: ship with blue ice. All other available sizes: ship with RT, or blue ice upon request. |
| General tips | For obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. Stock solution can be stored below -20°C for several months. |
Quality Control & MSDS
- View current batch:
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Purity = 98.00%
- COA (Certificate Of Analysis)
- MSDS (Material Safety Data Sheet)
Chemical structure
