DiD
DiD, also called DiDC18(5), is a red fluorescent probe that is widely used as a lipophilic tracker in living and fixed tissues and cells. DiD can insert into the membrane, and diffuse rapidly, staining the entire membrane surface red. DiD usually does not affect cell viability and development, so it is commonly used in cell tracking, such as anterograde and retrograde neuronal tracking. DiD is excited by the 633 nm He-Ne laser, and has longer excitation and emission wavelengths than DiI, providing an alternative for labeling cells and tissues that have significant intrinsic fluorescence.
DiD staining can be used in conjunction with immunofluorescence. It is recommended to use formaldehyde (PFA) for fixation after DiD staining. Meanwhile, if permeabilization is required, it is suggested to use Triton X-100 or digitonin. But permeabilization may affect the localization of DiD in the cell membrane. In addition, DiD has many other applications including detecting cell-cell fusion and adhesion, tracking cell migration, and labeling lipoproteins.
| Storage | Store at -20°C, away from light |
| M.Wt | 959.92 |
| Cas No. | 127274-91-3 |
| Formula | C61H99ClN2O4 |
| Solubility | ≥29.55 mg/mL in DMSO; ≥6.69 mg/mL in EtOH with ultrasonic; insoluble in H2O |
| Chemical Name | 2-((1E,3E)-5-((E)-3,3-dimethyl-1-octadecylindolin-2-ylidene)penta-1,3-dien-1-yl)-3,3-dimethyl-1-octadecyl-3H-indol-1-ium perchlorate |
| SDF | Download SDF |
| Canonical SMILES | CCCCCCCCCCCCCCCCCCN1C2=C(C(C)(C)/C1=C\C=C\C=C\C(C3(C)C)=[N+](CCCCCCCCCCCCCCCCCC)C4=C3C=CC=C4)C=CC=C2.O=Cl(=O)([O-])=O |
| Shipping Condition | Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice. |
| General tips | We do not recommend long-term storage for the solution, please use it up soon. |
Quality Control & MSDS
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Chemical structure
| Components | B8805-5 mg | B8805-10 mg |
| DiD | 5 mg | 10 mg |
The product should be stored at -20°C away from light and moisture. Stable for 1 year. The stock solution should be stored at -20°C away from light. Stable for 6 months. | ||