Myco-Clear Mycoplasma Removal Agent

Mycoplasmas are the smallest prokaryotic microorganism, only 0.1-0.3 μm in size. Due to their small size, mycoplasmas can penetrate rated filters (0.22~0.45 μm). Mycoplasma contamination remains a major problem in cell culture. Mycoplasmas can alter the DNA, RNA, and protein synthesis of culture cells, but they may not noticeably affect cell growth rates in many cases. And it is a must to eliminate mycoplasma contamination for cell culture. 

The commonly used mycoplasma removal reagents are mainly tetracycline, macrolide, and quinolone antibiotics. These antibiotics can only inhibit mycoplasma, but they do not kill mycoplasma directly, so they are slow to act and not long-lasting. Resistance strains can also be developed after prolonged use.

The main active ingredient of Myco-Clear Mycoplasma Removal Agent is a surfactant peptide extracted from fermented products of Bacillus subtilis, which can selectively bind to mycoplasma membranes and change the permeability of mycoplasma membranes, thereby directly killing mycoplasma. At the same time, it cannot cross the cell membrane of eukaryotic cells, so it does not affect the characteristics of the cell. During use, this reagent may affect the proliferative ability of cells, but the cells can return to their normal proliferative state after removing this reagent.

This reagent is a ready-to-use, sterile solution, and can eliminate mycoplasma in cells in 5-8 days. Compared with traditional antibiotic products, this reagent can directly kill mycoplasma, with better and more complete elimination, and the possibility of mycoplasma resistance is lower. For faster and simpler elimination of mycoplasma, we recommend using Myco-Clear Mycoplasma Removal Agent Plus (Cat. No. K2825), an upgraded version of this reagent.