Caspase-3 Colorimetric Assay Kit
Cysteine-dependent aspartate-directed proteases (Caspases) are a family of cysteine proteases that play important roles in apoptosis, necrosis, and inflammation. Sequential activation of caspases plays an important role in cell apoptosis. Caspase-3 is a caspase protein that cleaves and activates caspases 6 and 7, and is processed and activated by caspases 8, 9, and 10. Caspase-3 is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with Alzheimer's disease. Caspase-3 recognizes tetra-peptide sequences D-x-x-D and hydrolyzes peptide bonds after aspartic acid residues.
Caspase-3 Colorimetric Assay Kit provides a convenient and simple way for detecting the DEVD-dependent caspase activity. When cleavage of DEVD from the labeled substrate DEVD-p-nitroaniline (DEVD-pNA) by Caspase-3 or related caspases, the free pNA light emission can be quantified by using a microtiter plate reader or a spectrophotometer at 405 or 400 nm. Comparison of the absorbance of free pNA from an apoptotic sample with an uninduced control determines the fold increase in caspase-3 activity.
| Features | Fast and convenient Simple one-step procedure; takes 1-2 hours. Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in CPP32 activity. |
| Shipping | Gel pack |
| Storage Conditions | Store at -20°C. |
Related Biological Data
Related Biological Data
Related Biological Data
Related Biological Data
- 1. Dan Cao, et al. "Deficiency of immunoglobulin IgSF6 enhances antibacterial effects by promoting endoplasmic reticulum stress and the inflammatory response in intestinal macrophages." Mucosal Immunol. 2024 Feb 21:S1933-0219(24)00015-1. PMID: 38387824
- 2. Shouhua Wang, Ting ting Su, et al. "CircPVT1 promotes gallbladder cancer growth by sponging miR-339-3p and regulates MCL-1 expression." Cell Death Discov. 2021 Jul 26;7(1):191. PMID: 34312371
| Kit components | Cell Lysis Buffer 2X Reaction Buffer DEVD-pNA (4 mM) DTT (1 M) |
| Components | K2008-25 25 assays |
K2008-100 100 assays |
K2008-200 200 assays |
K2008-400 400 assays |
Part Number |
| Cell Lysis Buffer | 25 ml | 100 ml | 100 ml | 100 ml | K2008-C-1 |
| 2 X Reaction Buffer | 2 ml | 4 x 2 ml | 16 ml | 32 ml | K2008-C-2 |
| DEVD-pNA (4 mM) | 125 μl | 500 μl | 2 x 0.5 ml | 2 x 1 ml | K2008-C-3 |
| DTT (1 M) | 100 μl | 400 μl | 400 μl | 400 μl | K2008-C-4 |
Store at -20°C. | |||||