In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
CCT007093 is a small-molecule chemical inhibitor of PPM1D with IC50 value of 8.4μM .
CCT007093 is a thienylidene cyclopentanone and shows a potent inhibition of PPM1D in the in vitro assay when using the recombinant phospho-P38 as a substrate. In cellular assay, CCT007093 shows specificity for MCF-7 cells over HeLa cells. It reduces 40% viability of the cells after 2 days. It is found that the cell death induced by CCT007093 is dependent on P38 kinase activity. CCT007093 mimics the effect of PPM1D RNAi in activating P38 kinase. It induces P38 phosphorylation at 4 h post-exposure in MCF-7 cells and the loss of viability can be rescued by SB203580, the specific P38 inhibitor .
 Rayter S, Elliott R, Travers J, Rowlands MG, Richardson TB, Boxall K, Jones K, Linardopoulos S, Workman P, Aherne W, Lord CJ, Ashworth A. A chemical inhibitor of PPM1D that selectively kills cells overexpressing PPM1D. Oncogene. 2008 Feb 14;27(8):1036-44.
|Storage||Store at -20°C|
|Solubility||insoluble in EtOH; insoluble in H2O; ≥3.4 mg/mL in DMSO|
|Shipping Condition||Evaluation sample solution: ship with blue ice. All other available sizes: ship with RT, or blue ice upon request.|
|General tips||For obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. Stock solution can be stored below -20°C for several months.|
|Description||CCT007093 is a potent inhibitor of PPM1D (WIP1) with IC50 value of 8.4 μM.|