In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
BW-B 70C is a potent and selective inhibitor of 5-lipoxygenase .
5-lipoxygenase is an enzyme that transforms essential fatty acids (EFAs) into leukotrienes and is activated by 5-lipoxygenase activating protein (FLAP).
BW-B 70C is a potent and selective 5-lipoxygenase inhibitor. BW-B 70C had high potency and long duration in vivo and was considered as potential anti-asthma drug . In sensitised guinea-pigs, BW B70C (2-50 mg/kg) inhibited bronchoconstriction induced by allergen in a dose-dependent way, which was consistent with the inhibitory activity of BW B70C against 5-lipoxygenase. Also, BW B70C (20 mg/kg) inhibited eosinophil infiltration by 67% . In guinea-pigs, The 5-lipoxygenase inhibitor, BW B70C (30 mg/kg) inhibited leucocyte migration to the airways lumen, leukotriene C4 synthesis by alveolar macrophages and albumin microvascular leakage induced by endotoxin. However, BW B70C didn’t affect vascular leucocyte margination and the blood levels of secreted phospholipase A2 and TNF-α . In a focal cerebral ischemia rat model, BW-B 70C improved neurological deficit scores and decreased infarctions. Also, BW-B 70C reduced 5-lipoxygenase (5-LOX) and inhibited inducible nitric oxide synthase (iNOS) expression through down-regulation of NF-κB .
. Payne AN, Jackson WP, Salmon JA, et al. Hydroxamic acids and hydroxyureas as novel, selective 5-lipoxygenase inhibitors for possible use in asthma. Agents Actions Suppl, 1991, 34: 189-199.
. Yeadon M, Dougan FL, Petrovic A, et al. Effect of BW B70C, a novel inhibitor of arachidonic acid 5-lipoxygenase, on allergen-induced bronchoconstriction and late-phase lung eosinophil accumulation in sensitised guinea-pigs. Agents Actions, 1993, 38(1-2): 8-18.
. Bureau MF, De Castro CM, Cortese C, et al. 5-Lipoxygenase and endotoxin-induced microvascular albumin exchanges and leucocyte recruitment in guinea-pig lungs. Eur J Pharmacol, 1997, 324(1): 89-98.
. Jatana M, Giri S, Ansari MA, et al. Inhibition of NF-kappaB activation by 5-lipoxygenase inhibitors protects brain against injury in a rat model of focal cerebral ischemia. J Neuroinflammation, 2006, 3: 12.
|Physical Appearance||A crystalline solid|
|Storage||Desiccate at -20°C|
|Solubility||Limited solubility, soluble in DMSO or ethanol|
|Canonical SMILES||FC1=CC=C(C=C1)OC2=CC(/C=C/[[email protected]@H](C)N(C(N)=O)O)=CC=C2|
|Shipping Condition||Evaluation sample solution: ship with blue ice. All other available sizes: ship with RT, or blue ice upon request.|
|General tips||For obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. Stock solution can be stored below -20°C for several months.|