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Bromophenol Blue

Catalog No.
Electrophoretic/PH indicator,widely used in buffers
Grouped product items
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IC50: N/A

Bromophenol blue (3',3",5',5"-tetrabromophenolsulfonphthalein) is widely used in gel loading buffers. Bromophenol blue is experimentally used as acolor marker, apH indicator, and a dye. Bromophenol blue can be prepared by slowly adding excess bromine to a hot solution ofphenolsulfonphthaleinin the presence of glacial acetic acid.

In vitro: Previous results showed that the adsorption process of bromophenol blue increased with the increase in the concentration of α-chitin nanoparticles (CNP), contact time and temperature, while the adsorption process decreased with increase in the initial dye concentration and strong acidic pH. The Fourier transform infrared spectroscopy results confirmed that the interaction between Bromophenol blue and CNP involved physical adsorption. The adsorption process obeyed Langmuir isotherm and pseudo second order kinetics more effectively. The isotherm and kinetic models confirmed that CNP could be used as a suitable adsorbent material for the removal of dyestuff, such as Bromophenol blue, from effluents [1].

In vivo: So far, bromophenol blue has not been employd in animal in vivo study.

Clinical trial: N/A

[1] Dhananasekaran S,Palanivel R,Pappu S.  Adsorption of Methylene Blue, Bromophenol Blue, and Coomassie Brilliant Blue by α-chitin nanoparticles. J Adv Res.2016 Jan;7(1):113-24.

Chemical Properties

Physical AppearanceA solid
StorageStore at RT
Cas No.115-39-9
Solubility≥33.5 mg/mL in EtOH; insoluble in H2O; ≥11.52 mg/mL in DMSO with ultrasonic
Chemical Name3,3-bis(3,5-dibromo-4-hydroxyphenyl)-3H-benzo[c][1,2]oxathiole 1,1-dioxide
SDFDownload SDF
Canonical SMILESBrC(C=C(C=C1Br)C(C2=CC=CC=C32)(C(C=C4Br)=CC(Br)=C4O)OS3(=O)=O)=C1O
Shipping ConditionEvaluation sample solution: ship with blue ice. All other available sizes: ship with RT, or blue ice upon request.
General tipsFor obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. Stock solution can be stored below -20°C for several months.

Quality Control

Quality Control & MSDS

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Chemical structure

Bromophenol Blue