Blasticidin S HCl
Blasticidin S is an aminoacylnucleoside antibiotic originally discovered in Streptomyces griseochromogenes. It exhibits strong cytotoxic effects against both eukaryotic and prokaryotic systems, making it a powerful antimicrobial agent. Beyond its primary antibacterial and antifungal applications, blasticidin has also been reported to interfere with viral replication and suppress tumor cell proliferation, further expanding its utility as a research tool. Due to its broad-spectrum activity, it is frequently used as a selectable marker in cell biology. It efficiently selects for transformed cells harboring the bls, bsr, or BSD resistance genes.
The mechanism of blasticidin S involves direct inhibition of peptide bond formation during translation. By binding to the ribosomal large subunit, it stabilizes tRNA interaction and prevents the hydrolysis of peptidyl-tRNA, ultimately halting protein synthesis and inducing cell death. To establish optimal working conditions, it is suggested to perform a kill curve to identify the minimum concentration required to eliminate non-resistant cells.
| Physical Appearance | White solid |
| Storage | Store at -20°C |
| M.Wt | 458.9 |
| Cas No. | 3513-03-9 |
| Formula | C17H27ClN8O5 |
| Solubility | insoluble in DMSO; insoluble in EtOH; ≥52.4 mg/mL in H2O |
| Chemical Name | (2S,3S,6R)-3-[[(3R)-3-amino-5-[carbamimidoyl(methyl)amino]pentanoyl]amino]-6-(4-amino-2-oxopyrimidin-1-yl)-3,6-dihydro-2H-pyran-2-carboxylic acid;hydrochloride |
| SDF | Download SDF |
| Canonical SMILES | CN(CCC(CC(=O)NC1C=CC(OC1C(=O)O)N2C=CC(=NC2=O)N)N)C(=N)N.Cl |
| Shipping Condition | Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice. |
| General tips | We do not recommend long-term storage for the solution, please use it up soon. |
| Cell experiment:[1] | |
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Cell lines |
Mouse leukemia L5178Y cells |
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Reaction Conditions |
20 μg/ml blasticidin S for 6 h incubation |
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Applications |
In mouse leukemia L5178Y cells, blasticidin S resulted in a marked accumulation of monosome and ribosomal subunits, accompanied by a drastic decrease of pulse-labeled RNA (presumably mRNA) in the larger size region of polysomes. In response to a decreased protein synthesis (5 ~ 10% of the control), the appearance of pulse-labeled [3H]RNA among polysomes and monosome decreased to approximately 10% of the control when cells were treated with blasticidin S. |
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Note |
The technical data provided above is for reference only. |
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References: 1. Takenaka K, Endo H, Kuwano M. The synthesis and degradation of presumptive messenger RNA in cultured mouse leukemia cells during the inhibition of protein synthesis. Biochimica et Biophysica Acta, 1978, 521 (1): 295-307. |
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Quality Control & MSDS
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Chemical structure
