BAPTA-AM
BAPTA-AM, an acetoxymethyl ester derivative of BAPTA, is a membrane-permeant, highly selective calcium chelator. BAPTA-AM can be easily loaded into cells and hydrolyzed by intracellular esterases to convert into BAPTA. BAPTA-AM is ideal for real-time Ca²⁺ imaging, electrophysiology, synaptic transmission studies, and high-throughput drug screening.
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| Storage | Desiccate at -20°C |
| M.Wt | 764.68 |
| Cas No. | 126150-97-8 |
| Formula | C34H40N2O18 |
| Solubility | insoluble in H2O; insoluble in EtOH; ≥16.3 mg/mL in DMSO with gentle warming |
| SDF | Download SDF |
| Canonical SMILES | O=C(CN(CC(OCOC(C)=O)=O)C1=CC=CC=C1OCCOC2=CC=CC=C2N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)OCOC(C)=O |
| Shipping Condition | Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice. |
| General tips | We do not recommend long-term storage for the solution, please use it up soon. |
| Cell experiment [1, 2]: | |
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Cell lines |
Human leukemia cell lines HL-60 and U937, Bovine chromaffin cells |
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Preparation method |
The solubility of this compound in DMSO is >16.3mg/mL. General tips for obtaining a higher concentration: Please warm the tube at 37 ℃ for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below -20℃ for several months. |
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Reacting condition |
10 μM, 50 μM; 6 h; |
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Applications |
In human leukemia cell lines HL-60 and U937, BAPTA/AM (10 μM) exhibited classic apoptotic morphology, and BAPTA/AM (50 μM) induced atypical features (e.g., cell swelling, chromatin clumping).Superfusion with BAPTA-AM (50 μM) induced a rapid (< 60 s) and reversible block of both IKCa and IK (~50%). Preincubation with BAPTAAM (50 μM, 30 min) or cell loading with the non-permeable active form of BAPTA (10 mM in the pipette solution) permanently blocked IKCa. BAPTA-AM superfusion (50 μM) blocked IK (~53%) after BAPTA-loading or BAPTA-AM preincubation. |
| Animal experiment [3]: | |
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Animal models |
Swiss (RjOrl) mice, Male C57BL/6J mice |
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Dosage form |
0–10 mg/kg, 30 min |
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Application |
In Swiss (RjOrl) mice, pretreatment with BAPTA-AM (0–10 mg/kg, 30 min) prevented locomotor stimulation produced by ethanol without altering basal locomotion. BAPTA-AM reversed ethanol-induced hypnotic effects. Following a drinking-in-the-dark methodology, male C57BL/6J mice were offered 20% v/v ethanol, tap water, or 0.1% sweetened water. BAPTA-AM pretreatment (0–5 mg/kg) dose-dependently reduced ethanol consumption while leaving water and sweetened water intake unaffected. |
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Other notes |
Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal. |
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References: [1]. Grant S, Freemerman AJ, Gregory PC, et al. Induction of apoptotic DNA fragmentation and c-jun downregulation in human myeloid leukemia cells by the permeant Ca2+ chelator BAPTA/AM. Oncol Res, 1995, 7(7-8): 381-392. [2]. Urbano FJ, Buo W. BAPTA-AM blocks both voltage-gated and Ca2+-activated K+ currents in cultured bovine chromaffin cells. Neuroreport, 1998, 9(15): 3403-3407. [3] Balio P, Monferrer L, Pastor R, et al. Intracellular calcium chelation with BAPTA-AM modulates ethanol-induced behavioral effects in mice. Exp Neurol, 2012, 234(2): 446-453. | |
| Description | BAPTA-AM is a selective, membrane-permeable calcium chelator. | |||||
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Quality Control & MSDS
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