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BAPTA-AM

Catalog No.
B4758
A highly selective calcium chelator
Grouped product items
SizePriceStock Qty
10mM (in 1mL DMSO)
$83.00
In stock
10mg
$74.00
In stock
50mg
$261.00
In stock
For scientific research use only and should not be used for diagnostic or medical purposes.

Tel: +1-832-696-8203

Email: [email protected]

Worldwide Distributors

Background

BAPTA-AM, an acetoxymethyl ester derivative of BAPTA, is a membrane-permeant, highly selective calcium chelator. BAPTA-AM can be easily loaded into cells and hydrolyzed by intracellular esterases to convert into BAPTA. BAPTA-AM is ideal for real-time Ca²⁺ imaging, electrophysiology, synaptic transmission studies, and high-throughput drug screening. 

Product Citation

Chemical Properties

StorageDesiccate at -20°C
M.Wt764.68
Cas No.126150-97-8
FormulaC34H40N2O18
Solubilityinsoluble in H2O; insoluble in EtOH; ≥16.3 mg/mL in DMSO with gentle warming
SDFDownload SDF
Canonical SMILESO=C(CN(CC(OCOC(C)=O)=O)C1=CC=CC=C1OCCOC2=CC=CC=C2N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)OCOC(C)=O
Shipping ConditionSmall Molecules with Blue Ice, Modified Nucleotides with Dry Ice.
General tips We do not recommend long-term storage for the solution, please use it up soon.

Protocol

Cell experiment [1, 2]:

Cell lines

Human leukemia cell lines HL-60 and U937, Bovine chromaffin cells

Preparation method

The solubility of this compound in DMSO is >16.3mg/mL. General tips for obtaining a higher concentration: Please warm the tube at 37 ℃ for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below -20℃ for several months.

Reacting condition

10 μM, 50 μM; 6 h;

Applications

In human leukemia cell lines HL-60 and U937, BAPTA/AM (10 μM) exhibited classic apoptotic morphology, and BAPTA/AM (50 μM) induced atypical features (e.g., cell swelling, chromatin clumping).Superfusion with BAPTA-AM (50 μM) induced a rapid (< 60 s) and reversible block of both IKCa and IK (~50%). Preincubation with BAPTAAM (50 μM, 30 min) or cell loading with the non-permeable active form of BAPTA (10 mM in the pipette solution) permanently blocked IKCa. BAPTA-AM superfusion (50 μM) blocked IK (~53%) after BAPTA-loading or BAPTA-AM preincubation.

Animal experiment [3]:

Animal models

Swiss (RjOrl) mice, Male C57BL/6J mice

Dosage form

0–10 mg/kg, 30 min

Application

In Swiss (RjOrl) mice, pretreatment with BAPTA-AM (0–10 mg/kg, 30 min) prevented locomotor stimulation produced by ethanol without altering basal locomotion. BAPTA-AM reversed ethanol-induced hypnotic effects. Following a drinking-in-the-dark methodology, male C57BL/6J mice were offered 20% v/v ethanol, tap water, or 0.1% sweetened water. BAPTA-AM pretreatment (0–5 mg/kg) dose-dependently reduced ethanol consumption while leaving water and sweetened water intake unaffected.

Other notes

Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal.

References:

[1]. Grant S, Freemerman AJ, Gregory PC, et al. Induction of apoptotic DNA fragmentation and c-jun downregulation in human myeloid leukemia cells by the permeant Ca2+ chelator BAPTA/AM. Oncol Res, 1995, 7(7-8): 381-392.

[2]. Urbano FJ, Buo W. BAPTA-AM blocks both voltage-gated and Ca2+-activated K+ currents in cultured bovine chromaffin cells. Neuroreport, 1998, 9(15): 3403-3407.

[3] Balio P, Monferrer L, Pastor R, et al. Intracellular calcium chelation with BAPTA-AM modulates ethanol-induced behavioral effects in mice. Exp Neurol, 2012, 234(2): 446-453.

Biological Activity

Description BAPTA-AM is a selective, membrane-permeable calcium chelator.
Targets            
IC50            

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