In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
IC50: 8.9 μM: blocks AChE in human; 126 μM: inhibits acetylcholinesterase (AChE) in bovine; 160 μM: blocks muscarinic acetylcholine receptor M1, M2, M3 and M4 in mouse.
Asoxime, also known as HI-6, is an asymmetric bis-pyridinium aldoxime and reactivates AChE via nucleophilic attack of oximate anions on organophosphate-AChE conjugates.
AChE, one of the primary targets of organophosphates, is inactivated by organophosphates via reacting covalently with the active center serine, which blocks the hydrolyzation of acetylcholine at peripheral and central synapses. Additionally, the accumulation of acetylcholine leads to an over-stimulation of cholinergic receptors, which disrupts a variety of biological functions.
In vitro: HI-6 showed a bacteriostatic effect on Francisella tularensis (F. tularensis) and inhibited the growth of F. tularensis when treated with 100 mM HI-6 .
In vivo: Female BALB/c mice, infected by F. tularensis, were administrated subcutaneously with 100 μl HI-6 for ten days. The lower dose of HI-6 yielded in different symptoms and mortality. Compared to mice injected only with tularemia, groups of mice exposed to the lower dose of HI-6 showed a decline in mortality, which can be explained via higher efficacy of bacteriostatic effect while the toxic effect was decreased . Female Wistar rats were treated intramuscularly with HI-6 at a dose of 15.6 or 156 mg/kg for one day. The level of thiobarbituric acid reactive substances was increased, which was triggered by HI-62 .
. Pohanka, M., Pavli, O., Pikula, J., Treml, F., & Kuca, K. Modulation of Tularemia Disease Progress by the Bisquaternary Pyridinium Oxime HI-6. Acta Veterinaria Brno. 2010; 79(3): 443-448.
. Pohanka, M., Sobotka, J., Svobodova, H., & Stetina, R. Sulfur mustard induced oxidative stress and its alteration using asoxime (HI-6). Interdisciplinary Toxicology. 2013; 6(4).
|Physical Appearance||A crystalline solid|
|Storage||Store at -20°C|
|Solubility||≤10mg/ml in PBS(pH7.2)|
|Chemical Name||1-[[[4-(aminocarbonyl)pyridinio]methoxy]methyl]-2-[(hydroxyimino)methyl]-pyridinium, dichloride|
|Shipping Condition||Evaluation sample solution : ship with blue ice.All other available size: ship with RT , or blue ice upon request|
|General tips||For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.|