In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Allopregnanolone is a selective activator of GABA-A receptor with the concentration of 10 nM .
GABA-A receptor is a pentameric transmembrane receptor and sits in the membrane of neuron. As a subtype of receptor for the neurotransmitter GABA, GABA-A receptor mediates the bulk of synaptic inhibition in the central nervous system and prevents action potential generation by short-circuiting the depolarization produced by excitatory neurotransmission. It has been shown that allopregnanolone can directly activate GABA-A receptor and it also can allosteric enhance GABA-evoked currents to function  .
Allopregnanolone is an agonist for GABA-A receptor. When tested with rat retinas exposed to hydrostatic pressure (75 mm Hg) for 24 hours, administration of alloprenanolone significantly suppressed pressure-induced axonal swelling via functioning on GABA-A R in a dose-dependent manner .
In spinal cord slides from naïve rats, pre-incubation with TGOT significantly lower the following allopregnanolone (0.11 ± 0.04 ng/mg) treatment thus decayed GABA-A R which resulted in the spinal neurosteroidogenesis .
It is also reported that subcutaneously injected allopregnanolone to male Wistar rats once daily over five consecutive days resulted in energy intake and weight gain increasement .
. Ishikawa, M., et al., Neurosteroids are endogenous neuroprotectants in an ex vivo glaucoma model. Invest Ophthalmol Vis Sci, 2014. 55(12): p. 8531-41.
. Maguire, J. and I. Mody, Steroid hormone fluctuations and GABA(A)R plasticity. Psychoneuroendocrinology, 2009. 34 Suppl 1: p. S84-90.
. Juif, P.E., et al., Long-lasting spinal oxytocin analgesia is ensured by the stimulation of allopregnanolone synthesis which potentiates GABA(A) receptor-mediated synaptic inhibition. J Neurosci, 2013. 33(42): p. 16617-26.
. Holmberg, E., et al., Repeated allopregnanolone exposure induces weight gain in schedule fed rats on high fat diet. Physiol Behav, 2015. 140: p. 1-7.
|Physical Appearance||A crystalline solid|
|Storage||Store at RT|
|Solubility||Soluble in DMSO|
|Canonical SMILES||O[[email protected]]1C[[email protected]]2[[email protected]@](CC1)(C)[[email protected]@H]3[[email protected]@H](CC2)[[email protected]]4[[email protected]]([[email protected]@H](C(C)=O)CC4)(C)CC3|
|Shipping Condition||Evaluation sample solution: ship with blue ice. All other available sizes: ship with RT, or blue ice upon request.|
|General tips||For obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. Stock solution can be stored below -20°C for several months.|