In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
IC50: inhibits the phosphorylation of ERK1 and ERK2 at 25-50 μM
AG-126 is an ERK1 and ERK2 inhibitor.
Extracellular signal-regulated kinases (ERKs) are widely expressed intracellular signalling molecules that are involved in functions including the regulation of meiosis, mitosis, and postmitotic functions. Many different stimuli, such as growth factors, cytokines, transforming agents, and carcinogens, can activate the ERK pathway.
In vitro: Previous study found that r AG126 ould potently inhibit the PCW-evoked release and this effect was rather selective with respect to the stimulus. AG126 was found to be less potent in attenuating LPS-triggered release. Moreover, it was found that AG126 was able to prevent the PCW-inducible ERK phosphorylation, which could relate to the AG126-mediated inhibition of PCW-evoked cytokine release .
In vivo: AG126 was tested in a model of PCW-induced meningitis, in which rats underwent intracisternal injection of PCW with and without AG126 pretreatment. PCW injection led to a dramatic leukocyte influx into the CSF. AG126 treatment could significantly attenuate such invasion, without changing the number of circulating leukocytes. In addition, AG126 treatment showed beneficial effects on other parameters, such as intracranial pressure. Furthermore, AG126 had no adverse effect on several physiological parameters including mean arterial blood pressure, arterial PaO2, PaCO2 as well as pH .
Clinical trial: So far, no clinical study has been conducted.
 Hanisch, U. K.,Prinz, M.,Angstwurm, K., et al. The protein tyrosine kinase inhibitor AG126 prevents the massive microglial cytokine induction by pneumococcal cell walls. European Journal of Immunology 31, 2104-2115 (2001).
|Physical Appearance||A crystalline solid|
|Storage||Store at -20°C|
|Solubility||≤0.15mg/ml in ethanol;10mg/ml in DMSO;10mg/ml in dimethyl formamide|
|Shipping Condition||Evaluation sample solution : ship with blue ice.All other available size: ship with RT , or blue ice upon request|
|General tips||For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.|