6-Hydroxydopamine hydrobromide

6-Hydroxydopamine Hydrobromide (CAS 636-00-0) is a catecholaminergic neurotoxin widely used to induce dopaminergic lesions and Parkinson's disease symptoms in rodent models of Parkinson's disease.

Injection of 6-OHDA at doses of 9 microg, 6 microg, and 18 microg into the substantia nigra, medial forebrain bundle (MFB), and striatum, respectively, causes motor dysfunction in mice and a significant reduction in tyrosine hydroxylase-immunoreactive neurons in the brain. Intracisternal administration to rats (200 microg 6-OHDA) reduces brain dopamine and norepinephrine levels. In addition, 6-OHDA at 50 micromol/L induces apoptosis in primary rat cortical cells and rat adrenal PC12 cells. At 75 micromol/L, 6-OHDA time-dependently activates caspases-3, -8, and -9, elevates intracellular reactive oxygen species (ROS) levels in PC12 cells; and when this concentration of 6-OHDA is stereotactically injected into the right MFB of rats, it induces apoptosis in the substantia nigra.

References

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