6-Hydroxydopamine hydrobromide

6-OHDA (CAS 28094-15-7)is a catecholaminergic neurotoxin widely utilized to induce dopaminergic lesions and parkinsonian symptoms in rodent models of Parkinson’s disease. Administration of 6-OHDA into the substantia nigra, medial forebrain bundle (MFB), or striatum at doses of 9, 6, and 18 μg, respectively, results in locomotor impairment in mice and a significant loss of tyrosine hydroxylase-immunoreactive neurons in the mouse brain. Intracisternal administration of 6-OHDA (200 μg) has been shown to decrease both dopamine and norepinephrine levels in the rat brain. Furthermore, 6-OHDA induces apoptosis in primary rat cortical cells and PC12 rat adrenal cells at a concentration of 50 μM. At a concentration of 75 μM, 6-OHDA activates caspase-3, -8, and -9 in a time-dependent manner, increases intracellular reactive oxygen species (ROS) levels in PC12 cells, and induces apoptosis in the rat substantia nigra when stereotaxically injected into the right MFB.

References:

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