6-Bnz-cAMP sodium salt
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
6-Bnz-cAMP is a PKA-selective activator. It regulates the PKA dependent signaling pathways.
Like PKA contains an evolutionally conserved cAMP-binding domain that acts as a molecular switch for sensing intracellular second messenger cAMP levels to control diverse biological functions. CAMP response element-binding protein (CREB) is the well-known direct target protein of PKA. Phosphorylation of CREB (pCREB) by PKA has been shown to be involved in regulating osteoblast differentiation.
The proliferative signaling pathway which activated by the 6-Bnz-cAMP involves activation of the epidermal growth factor receptor and ERK1/2. Extending the duration of PKA-dependent ERK1/2 activation and converted cAMP from a proliferative into an anti-proliferative, neurite outgrowth- promoting signal.
6-Bnz-cAMP can promote not only differentiation and mineralization, but also initial cell adhesion.6-Bnz-cAMP is able to induce osteogenic differentiation of MC3T3-E1 cells. Moreover 6-Bnz-cAMP may facilitate release kinetic from a tissue-engineered polymeric scaffold system. It also can serve as a novel bone-inducing growth factor for repairing and regenerating bone tissues during bone regenerative engineering.
Lo KW, Kan HM, Ashe KM, Laurencin CT. The small molecule PKA-specific cyclic AMP analogue as an inducer of osteoblast-like cells differentiation and mineralization. J Tissue Eng Regen Med. 2012 Jan;6(1):40-8.
Simone Kiermayer, Ricardo M. Biondi,etal., Epac Activation Converts cAMP from a Proliferative into a Differentiation Signal in PC12 Cells. Molecular Biology of the Cell. Vol. 16, 5639–5648, December 2005.
Cheng X, Ji Z, Tsalkova T, Mei F. Epac and PKA: a tale of two intracellular cAMP receptors. Acta Biochim Biophys Sin (Shanghai). 2008 Jul;40(7):651-62.
|Physical Appearance||A crystalline solid|
|Storage||Store at -20°C|
|Solubility||Soluble in H2O|
|Chemical Name||sodium (4aR,6R,7R,7aS)-6-(6-benzamido-9H-purin-9-yl)-7-hydroxytetrahydro-4H-furo[3,2-d][1,3,2]dioxaphosphinin-2-olate 2-oxide|
|Canonical SMILES||O=C(NC1=NC=NC2=C1N=CN2[[email protected]@H]3O[[email protected]@](COP(O4)(O[Na])=O)([H])[[email protected]]4([H])[[email protected]]3O)C5=CC=CC=C5|
|Shipping Condition||Evaluation sample solution : ship with blue ice.All other available size: ship with RT , or blue ice upon request|
|General tips||For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.|