Index Kit 2 for Illumina (384)
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
| Components | 384 rxns | Bases in adapter | Index Sequence (MiSeq, NovaSeq, Hiseq 2000/2500) |
Index Sequence (MiSeq, NovaSeq, Hiseq 3000/4000) |
| N601 | 240 μL | CTCTCTAT | CTCTCTAT | ATAGAGAG |
| N602 | 240 μL | TATCCTCT | TATCCTCT | AGAGGATA |
| N603 | 240 μL | GTAAGGAG | GTAAGGAG | CTCCTTAC |
| N604 | 240 μL | ACTGCATA | ACTGCATA | TATGCAGT |
| N605 | 240 μL | AAGGAGTA | AAGGAGTA | TACTCCTT |
| N606 | 240 μL | CTAAGCCT | CTAAGCCT | AGGCTTAG |
| N607 | 240 μL | CGTCTAAT | CGTCTAAT | ATTAGACG |
| N608 | 240 μL | TCTCTCCG | TCTCTCCG | CGGAGAGA |
| N609 | 240 μL | TCGACTAG | TCGACTAG | CTAGTCGA |
| N610 | 240 μL | TTCTAGCT | TTCTAGCT | AGCTAGAA |
| N611 | 240 μL | CCTAGAGT | CCTAGAGT | ACTCTAGG |
| N612 | 240 μL | GCGTAAGA | GCGTAAGA | TCTTACGC |
| N613 | 240 μL | CTATTAAG | CTATTAAG | CTTAATAG |
| N614 | 240 μL | CTATTAAG | CTATTAAG | CTTAATAG |
| N615 | 240 μL | GAGCCTTA | GAGCCTTA | TAAGGCTC |
| N616 | 240 μL | TTATGCGA | TTATGCGA | TCGCATAA |
Store the components at -20°C for one year. | ||||
| Components | 384 rxns | Bases in adapter | Index Sequence |
| N801 | 160 μL | TCGCCTTA | TAAGGCGA |
| N802 | 160 μL | CTAGTACG | CGTACTAG |
| N803 | 160 μL | TTCTGCCT | AGGCAGAA |
| N804 | 160 μL | GCTCAGGA | TCCTGAGC |
| N805 | 160 μL | AGGAGTCC | GGACTCCT |
| N806 | 160 μL | CATGCCTA | TAGGCATG |
| N807 | 160 μL | GTAGAGAG | CTCTCTAC |
| N808 | 160 μL | CAGCCTCG | CGAGGCTG |
| N809 | 160 μL | TGCCTCTT | AAGAGGCA |
| N810 | 160 μL | TCCTCTAC | GTAGAGGA |
| N811 | 160 μL | TCATGAGC | GCTCATGA |
| N812 | 160 μL | CCTGAGAT | ATCTCAGG |
| N813 | 160 μL | TAGCGAGT | ACTCGCTA |
| N814 | 160 μL | GTAGCTCC | GGAGCTAC |
| N815 | 160 μL | TACTACGC | GCGTAGTA |
| N816 | 160 μL | AGGCTCCG | CGGAGCCT |
| N817 | 160 μL | GCAGCGTA | TACGCTGC |
| N818 | 160 μL | CTGCGCAT | ATGCGCAG |
| N819 | 160 μL | GAGCGCTA | TAGCGCTC |
| N820 | 160 μL | CGCTCAGT | ACTGAGCG |
| N821 | 160 μL | GTCTTAGG | CCTAAGAC |
| N822 | 160 μL | ACTGATCG | CGATCAGT |
| N823 | 160 μL | TAGCTGCA | TGCAGCTA |
| N824 | 160 μL | GACGTCGA | TCGACGTC |
Store the components at -20°C for one year. | |||
Index Kit 2 for Illumina (384) is a dedicated primer kit for Tn5 DNA Library Prep Kit for Illumina (Cat. No. K1055, K1056, K1057). It contains 16 kinds of N6XX and 24 kinds of N8XX that can be used to prepare 384 different dual-indexed adapter combinations. Index Kit 2 for Illumina (384) is equivalent to Nextera XT Index Kit v2 (Illumina, Cat. No. FC-131-2001, FC-131-2002, FC-131-2003, FC-131-2004). The prepared library was suitable for sequencing on the illumina platform such as HiSeq 2000/2500/3000/4000 and MiniSeq.
All reagents in the kit undergo rigorous quality control and functional verification to maximize the stability and repeatability of library construction.
1. It contains 16 kinds of N6XX and 24 kinds of N8XX that can be used to prepare 384 different dual-indexed adapter combinations.
2. All reagents in the kit undergo rigorous quality control and functional verification
