Cynomolgus HGF / Hepatocyte Growth Factor Protein
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
A DNA sequence encoding the cynomolgus HGF (C9E9X5) (Met1-Ser728) was expressed.
|Expression Host||Human Cells|
|Purity||(49.5+35.5+6.4+7.1) % as determined by SDS-PAGE|
|Bio-activity||1. Immobilized Cynomolgus HGF at 10 μg/ml (100 μl/well) can bind Cynomolgus MET-Fc (cat:90304-C02H), EC50 of Cynomolgus MET-Fc (cat:90304-C02H) is 0.04-0.09 μg/ml.
2. Measured by its ability to inhibit TGF?1 activity on Mv-1-lu mink lung epithelial cells. The ED50 for this effect is typically 2-10 ng/ml.
|Endotoxin||< 1.0 EU per μg of the protein as determined by the LAL method|
|Stability||Samples are stable for up to twelve months from date of receipt at -70℃|
|Predicted N terminal||His 32|
The recombinant cyno HGF consists of 697 amino acids and has a calculated molecular mass of 79.6 KDa. It migrates as 89,58,34 and 32 KDa band in SDS-PAGE under reducing conditions.
|Formulation||Lyophilized from sterile PBS, pH 7.4.
|Storage||Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.|
|Reconstitution||A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.|
|HGF / Hepatocyte Growth Factor Protein Description||
Hepatocyte growth factor (HGF), also known as Scatter factor, is a paracrine cellular growth, motility and morphogenic factor. Hepatocyte Growth Factor/HGF is a member of the peptidase S1 family, and is derived from a single chain precursor of 728 amino acid residues containing 4 kringle domains, 1PAN domain and 1peptidase S1 domain. The synthesized single chain of HGF is further processed into a bioactive heterodimer composed of the 69 kDa α-subunit and the 34 kDa β-subunit by a serine protease. Hepatocyte Growth Factor/HGF has no detectable protease activity. Alternative splicing of its gene produces multiple transcript variants encoding different isoforms. It is secreted by mesenchymal cells and targets and acts primarily upon epithelial cells and endothelial cells, as well as haemopoietic progenitor cells. Hepatocyte Growth Factor/HGF regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the c-Met receptor. Hepatocyte Growth Factor/HGF may also play an important role as a paracrine or endocrine mediator through an epithelial-mesenchymal interaction in wound-healing, tissue or organ regeneration, morphogenesis and carcinogenesis.