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ApoBrdU DNA Fragmentation Assay Kit

Catalog No.
K2070
Detects DNA Fragmentation by FACS or FL.
Grouped product items
SizePriceStock Qty
60 assays
$618.00
In stock

Tel: +1-832-696-8203

Email: [email protected]

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Background

Internucleosomal DNA fragmentation is a sign of apoptosis in mammalian cells. The BrdU In Situ DNA Fragmentation Assay Kit provides a simple and convenient way for detection of DNA fragmentation in biological samples based on fluorescence microscopy or flow cytometry method. The kit provides complete components including positive and negative control cells. The assay uses Br-dUTP (bromolated deoxyuridine triphosphate nucleotides) which is more easily incorporated into DNA strand breaks than other larger ligands such as digoxigenin, fluorescein and biotin. The greater incorporation generates brighter signal and the Br-dUTP sites can be identified by a fluorescein labeled anti-BrdU monoclonal antibody.

Features & Properties

FeaturesFast and convenient Simple procedure; takes ~ 1 hour The assay is sensitive and stable. The incorporation of BrdU in the procedure provides greater sensitivity than biotinylated or digoxigenylated methods.
ShippingGel pack
Storage ConditionsStore at -20℃.

Quality Control

Quality Control & DataSheet

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Related Biological Data

ApoBrdU DNA Fragmentation Assay Kit

Related Biological Data

ApoBrdU DNA Fragmentation Assay Kit

Related Biological Data

ApoBrdU DNA Fragmentation Assay Kit
 

Overview

Kit componentsPositive Control Cells Negative Control Cells Wash Buffer Reaction Buffer TdT Enzymes Br-dUTP Rinse Buffer Anti-BrdU-FITC Antibody PI/RNase Staining Buffer