ApoBrdU-IHC DNA Fragmentation Assay Kit

Internucleosomal DNA fragmentation is a sign of apoptosis in mammalian cells. The ApoBrdU-IHC DNA Fragmentation Assay Kit provides a simple and convenient way for detection of DNA fragmentation in fixed cell preparations or tissue sections based on immunohistochemistry method. The assay provides a two-color TUNEL (Terminal deoxynucleotide transferase dUTP Nick End Labeling) assay to label DNA fragmentation and then detect apoptotic cells by immunohistochemistry. The kit provides complete components including positive and negative control slides for assessing reagent performance; terminal deoxynucleotidyl tranferase enzyme (TdT), proteinase K, bromodeoxyuridine triphosphate (Br-dUTP), biotin labeled anti-BrdU antibody for labeling DNA fragmentation; reaction and blocking buffers for processing individual steps; horseradish peroxidase streptavidin conjugate, H₂O₂/Urea Tablets, DAB Tablets for color production and methyl green solution for counter staining the cells.