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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Tumor Necrosis Factor-α (TNF-α) is a potent cytokine expressed as a 26 kDa transmembrane protein with 233 aa that is cleaved to the soluble 17 kDa monomer by TNF-α converting enzyme (TACE) and forms stable homotrimers. TNF-α is expressed in many cells and plays an important role in inflammation, immune modulation, septic shock, viral replication, antitumor activity, anorexia, cachexia and hematopoiesis. TNF-α is chiefly produced by macrophages when challenged with viruses or bacteria. TNF-α is cytotoxic for many tumor cells lines but induced activation, differentiation or proliferation in normal cells. Tumor necrosis factor receptor TNFR1 and TNFR2 are receptors for TNF-α.
Cell lines
Human neuroblastoma SH-SY5Y cells
Reaction Conditions
20 ng/ml TNF-α for 24 h incubation
Applications
TNF-α upregulated CXCR4 expression in neuroblastoma cells and increased migration to the CXCR4 ligand SDF‑1α. In addition, this effect was dependent upon NF-κB transcriptional activity, as blocking the NF‑κB pathway with pyrrolidinedithiocarbamic acid ammonium salt suppressed TNF‑α‑induced upregulation of CXCR4 expression and reduced the migration towards the CXCR4 ligand, SDF‑1α. These findings indicate that the inflammatory cytokine, TNF‑α, partially functions through the NF‑κB signaling pathway to upregulate CXCR4 expression to foster neuroblastoma cell metastasis.
Note
The technical data provided above is for reference only.
References:
1. Zhi Y, Lu H, Duan Y, et al. Involvement of the nuclear factor-κB signaling pathway in the regulation of CXC chemokine receptor-4 expression in neuroblastoma cells induced by tumor necrosis factor-α. International Journal of Molecular Medicine, 2015, 35(2): 349-357.