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Recombinant Human IL-1 alpha

Catalog No.
P1120
Interleukin-1 alpha (IL-1α) is also known as IL-1A, IL1, and is a cytokine of the interleukin-1 family.
Grouped product items
SizePriceStock Qty
10ug
$246.00
Ship with 5-10 days
100ug
$1,165.00
Ship with 5-10 days
500ug
$2,614.00
Ship with 5-10 days
For scientific research use only and should not be used for diagnostic or medical purposes.

Tel: +1-832-696-8203

Email: [email protected]

Worldwide Distributors

Background

Interleukin-1 alpha (IL-1α) is a non-secreted proinflammatory cytokine produced mainly by activated macrophages, as well as neutrophils, epithelial cells, and endothelial cells. It possesses metabolic, physiological, haematopoietic activities, and plays one of the central roles in the regulation of the immune responses. Both IL-1α and IL-1β binds to the same receptor and has similar identical biological properties. Among various species, the amino acid sequence of mature IL-1α is conserved 60 % to 70 % and human IL-1 has been found to be biologically active on murine cell lines. IL-1α recently started to find effective application in cosmetic and dermatological formulations, which allow to significantly harmonizing derma architecture. 

Reference:

1. Bankers-Fulbright, J.L., K.R. Kalli, and D.J. McKean. 1996. Life Sci, 59: 61-83.

2. Dinarello, C.A. 1997. Semin Oncol, 24: S9-81-S9-93.

Description

Gene ID

3552

Accession #

P01583

Alternate Names

Interleukin-1 alpha, IL-1α, BAF, IL-1F1, LAF, LEM, preinterleukin 1 alpha, pro-interleukin-1-alpha

Source

Escherichia coli.

M.Wt

Approximately 18.0 kDa, a single non-glycosylated polypeptide chain containing 159 amino acids.

AA Sequence

SAPFSFLSNV KYNFMRIIKY EFILNDALNQ SIIRANDQYL TAAALHNLDE AVKFDMGAYK SSKDDAKITV ILRISKTQLY VTAQDEDQPV LLKEMPEIPK TITGSETNLL FFWETHGTKN YFTSVAHPNL FIATKQDYWV CLAGGPPSIT DFQILENQA

Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

- 12 months from date of receipt, -20 to -70 °C as supplied.

- 1 month, 2 to 8 °C under sterile conditions after reconstitution.

- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Formulation

Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4.

Solubility

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine D10S cells is less than 1.0 pg/ml, corresponding to a specific activity of > 1.0 × 109 IU/mg.

Shipping Condition

Gel pack.

Handling

Centrifuge the vial prior to opening.

Usage

For Research Use Only! Not to be used in humans.

Quality Control

Quality Control & DataSheet

View current batch:
    Purity: > 97% by SDS-PAGE and HPLC analyses.
    Endotoxin: Less than 1.0 EU/µg of rHuIL-1α as determined by LAL method.
  • Datasheet