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Recombinant Human IL-1beta/IL-1F2

Catalog No.
PH2002
Recombinant Human IL-1beta/IL-1F2
Grouped product items
SizePriceStock Qty
10ug
$163.00
In stock
100ug
$633.00
In stock
For scientific research use only and should not be used for diagnostic or medical purposes.

Tel: +1-832-696-8203

Email: [email protected]

Worldwide Distributors

Background

Interleukin-1 beta (IL1 beta or IL1B) also known as catabolin, is a member of the interleukin 1 cytokine family. IL1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 21% amino acid (aa) identity in human. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1R accessory protein (IL-1R3/IL-1RAcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. The human IL-1 beta cDNA encodes a 269 aa precursor. A 116 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta converting enzyme (Caspase-1/ICE) to generate the active cytokine [5-7]. The 17 kDa mature human IL-1 beta shares 96% aa sequence identity with rhesus and 67%-78% with canine, cotton rat, equine, feline, mouse, porcine, and rat IL-1 beta.

Reference

[1]. Allan, S.M. et al. (2005) Nat. Rev. Immunol. 5:629.

[2]. Boraschi, D. and A. Tagliabue (2006) Vitam. Horm. 74:229.

[3]. Kornman, K.S. (2006) Am. J. Clin. Nutr. 83:475S.

[4]. Isoda, K. and F. Ohsuzu (2006) J. Atheroscler. Thromb. 13:21.

[5]. March, C.J. et al. (1985) Nature 315:641.

[6]. Auron, P.E. et al. (1984) Proc. Natl. Acad. Sci. 81:7907.

[7]. Martinon, F. and J. Tschopp (2007) Cell Death Differ. 14:10..

Description

Accession #

P01584

Alternate Names

Human IL1 beta; IL-1 beta; IL-1; IL-1b; IL1-BETA; IL1F2; IL-1 beta; interleukin-1 beta

Source

Human embryonic kidney cell, HEK293-derived human IL1-beta protein

Protein sequence

Ala117-Ser269

M.Wt

17.4 kDa

Appearance

Solution protein.

Stability & Storage

Avoid repeated freeze-thaw cycles. It is recommended that the protein be aliquoted for optimal storage. 12 months from date of receipt, -20 to -70 °C as supplied.

Concentration

0. 2 mg/mL

Formulation

Dissolved in sterile PBS buffer.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. This solution can be diluted into other aqueous buffers.

Biological Activity

Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The EC50 for this effect is 1-10 pg/mL.

Shipping Condition

Shipping with dry ice.

Handling

Centrifuge the vial prior to opening.

Usage

For Research Use Only! Not to be used in humans.

 

Quality Control

Quality Control & MSDS

View current batch:
    Purity > 95%, determined by SDS-PAGE.
    Endotoxin: <0.010 EU per 1 ug of the protein by the LAL method.
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