Tn5 DNA Library Prep Kit for Illumina (for 5 ng DNA)
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
| Components | 24 rxns | 96 rxns |
| Transposase Mix | 120 uL | 480 uL |
| 2xTagmetation Buffer | 240 uL | 960 uL |
| 5xStop Solution | 120 uL | 480 uL |
| Amplify Enzyme | 24 uL | 96 uL |
| 5xAmplify Buffer | 240 uL | 960 uL |
| dNTP | 96 uL | 384 uL |
| Control DNA | 10 uL | 10 uL |
Control DNA, Escherichia coli Genomic DNA, 50 ng/uL | ||
Tn5 DNA Library Prep Kit for Illumina (for 5 ng DNA) is an optimized genomic library construction kit for the Illumina high-throughput sequencing platform, which is suitable for the amount of 5 ng input DNA. Traditional library construction generally includes multi-step operations such as DNA fragmentation, end repair and linker ligation, while our kit adopts Tn5 transposase method which can be completed in one-step reaction. Our kits are easy to operate and greatly reduce library construction time, and significantly reducing the demanded amount of the initial DNA.
The Transposase Mix and buffers provided by the kit are subject to rigorous quality control and functional verification, so as to achieve high fidelity, stability and repeatability.
The kit is suitable for a variety of purified DNA samples including human, animal, plant, microbial genomes and PCR products. When using PCR products for library preparation, make sure the size of PCR products is > 500 bp. The transposase is not effective at the ends of DNA, to avoid the reduction of sequencing coverage on the ends, it is recommended to extend 50-100 bp at the both ends of the to-be-sequenced region when PCR products are prepared.
Overview of the Workflow
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6. Kitagawa Y, Ohkura N, Kidani Y, et al. Guidance of regulatory T cell development by Satb1-dependent super-enhancer establishment. Nat Immunol. 2017; 18(2):173–183.
7. Marie R, Pedersen JN, Bærlocher L, et al. Single-molecule DNA-mapping and whole-genome sequencing of individual cells. Proc Natl Acad Sci U S A. 2018; 115(44):11192–11197.
8. Tassetto M, Kunitomi M, Whitfield ZJ, et al. Control of RNA viruses in mosquito cells through the acquisition of vDNA and endogenous viral elements. Elife. 2019; 8:e41244.
1. One tube enzymatic reaction, easy to operate
2. A single sample library process takes only 90 minutes
3. Sample input DNA volume can be as low as 5 ng, high library conversion efficiency
4. The Transposase Mix and buffers provided by the kit are subject to rigorous quality control and functional verification, so as to achieve high fidelity, stability and repeatability.