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Luciferase Reporter Assay Kit

Catalog No.
K2181
 
Grouped product items
SizePriceStock Qty
200 assays
$114.00
In stock

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Background

Firefly luciferase catalyzes the highly efficient oxidative carboxylation of luciferin. The light emission of this biolumninescence reaction can be detected by a luminometer. Firefly luciferase is a sensitive reporter for gene regulation and function study. The Luciferase Reporter Assay Kit offers a fast and easy way of sensitive way of detecting lucifeasue activity in transfected eukaryotic cells. For measurement of expressed luciferase in vitro, luciferase is first extracted from transfected cells through cell lysis. Then CoA, ATP, Mg²ᶧ and buffer are added to the lysate. Then the injection of luciferin triggers the luminescent reaction which the emitted light can be detected by luminometer. This Luciferase Reporter Assay includes CoA, ATP and Mg²ᶧ in an optimized buffer solution, to ensure highest sensitivity, consistent light output , plus convenience and consistency for multiple samples.

Features & Properties

FeaturesSimple procedure; takes ~ 20-30 minutes Fast and convenient The assay ensures maximal sensitivity, consistent light output, as well as accuracy and consistency when working with multiple samples.
ShippingGel pack
Storage ConditionsStore at -20°C.

Quality Control

Quality Control & DataSheet

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Overview

Kit componentsSubstrate A Substrate B Cell Lysis Buffer