Luciferase Reporter Assay Kit
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
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Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Firefly luciferase catalyzes the highly efficient oxidative carboxylation of luciferin. The light emission of this biolumninescence reaction can be detected by a luminometer. Firefly luciferase is a sensitive reporter for gene regulation and function study. The Luciferase Reporter Assay Kit offers a fast and easy way of sensitive way of detecting lucifeasue activity in transfected eukaryotic cells. For measurement of expressed luciferase in vitro, luciferase is first extracted from transfected cells through cell lysis. Then CoA, ATP, Mg²ᶧ and buffer are added to the lysate. Then the injection of luciferin triggers the luminescent reaction which the emitted light can be detected by luminometer. This Luciferase Reporter Assay includes CoA, ATP and Mg²ᶧ in an optimized buffer solution, to ensure highest sensitivity, consistent light output , plus convenience and consistency for multiple samples.
Features & Properties
|Features||Simple procedure; Takes approx. 20-30 minutes; Fast and convenient; The assay ensures maximal sensitivity, consistent light output, as well as accuracy and consistency when working with multiple samples.|
|Storage Conditions||Store at -20°C.|
Quality Control & DataSheet
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|Kit components||Substrate A, Substrate B, Cell Lysis Buffer|