In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
α-MSH is a 13-amino acid neuropeptide obtained from the precursor proopiomelanocortin (POMC), which is a vital stress hormone of the hypothalamus/pituitary/adrenal gland (HPA) axis .
Five G-protein-coupled melanocortin receptors (MC1-R to MC5-R) can transmit the signaling of melanocortins including α-MSH, which can mediate the effect of α-MSH on melanogenesis, thermoregulation, pigmentation formation, food intake, energy expenditure, and fatty acid oxidation.
In α-MSH-treated human umbilical vein endothelial cells (HUVECs), the blocking either MC1-R or MC2-R restored the Nitric Oxide release by using the antibody neutralization strategy. After immunoblot analysis, the MC1-R neutralization could inhibit the α-MSH-induced endothelial nitric oxide synthase (eNOS) and inducible nitric oxide synthase (iNOS) signaling, while MC2-R neutralization could reverse the α-MSH-induced eNOS but not iNOS signaling in HUVECs .
After treatment with α-MSH with or without L-Arginine for 24 h, it showed that α-MSH treatment potently interfered with the vessel development in transgenic zebrafish, L-Arginine supplementation rescued the α-MSH-induced intersegmental vessels (ISV) and restored endothelial recruitment in ISV .
 Weng W T, Wu C S, Wang F S, et al. α-Melanocyte-Stimulating Hormone Attenuates Neovascularization by Inducing Nitric Oxide Deficiency via MC-Rs/PKA/NF-κB Signaling [J]. International Journal of Molecular Sciences. 2018, 19(12): 3823.
|Physical Appearance||White lyophilised solid|
|Storage||Desiccate at -20°C|
|Solubility||Soluble to 0.60 mg/ml in sterile water|
|Canonical SMILES||CC([[email protected]@](/N=C(O)/[[email protected]]1([H])CCCN1C([[email protected]](/N=C(O)/C/N=C(O)/[[email protected]](/N=C(O)/[[email protected]](/N=C(O)/[[email protected]](/N=C(O)/[[email protected]](/N=C(O)/[[email protected]](/N=C(O)/[[email protected]](/N=C(O)/[[email protected]](/N=C(O)/[[email protected]](/N=C(O)/[[email protected]](/N=C(O)/C)([H])CO)([H])CC2=CC=C(O)C=C2)([H])CO)([H])CCSC)([H])CCC(O)=O)([H])CC3=CN=CN3)([H|
|Shipping Condition||Evaluation sample solution: ship with blue ice. All other available sizes: ship with RT, or blue ice upon request.|
|General tips||For obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. Stock solution can be stored below -20°C for several months.|