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GenuinePrep DNA Library Prep Kit for Illumina (for 1 ng DNA)

Catalog No.
K1055
Direct and rapid library construction of purified DNA samples by transposase
Grouped product items
SizePriceStock Qty
24 rxns
$558.00
In stock
96 rxns
$2,160.00
In stock

Tel: +1-832-696-8203

Email: [email protected]

Worldwide Distributors

Quality Control

Quality Control & DataSheet

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Components and Storage

Components 24 rxns 96 rxns
Transposase Mix 120 μL 480 μL
2X Tagmetation Buffer 240 μL 960 μL
5X Stop Solution 120 μL 480 μL
Amplify Enzyme 24 μL 96 μL
5X Amplify Buffer 240 μL 960 μL
dNTP Mixture 96 μL 384 μL
Control DNA 10 μL 10 μL

Store 5X Stop Solution at room temperature, Transposase Mix at 4°C and other components at -20°C.
*Note: Control DNA, Escherichia coli Genomic DNA, 50 ng/μL.

Description

GenuinePrep DNA Library Prep Kit for Illumina (for 1 ng DNA) is an optimized genomic library construction kit for the Illumina high-throughput sequencing platform, which is suitable for the amount of 1 ng input DNA. Traditional library construction generally includes multi-step operations such as DNA fragmentation, end repair and linker ligation, while our kit adopts GenuinePrep Tn5 transposase method which can be completed in one-step reaction. Our kits are easy to operate and greatly reduce library construction time, and significantly reducing the demanded amount of the initial DNA.

The Transposase Mix and buffers provided by the kit are subject to rigorous quality control and functional verification, so as to achieve high fidelity, stability and repeatability.

The kit is suitable for a variety of purified DNA samples including human, animal, plant, microbial genomes and PCR products. When using PCR products for library preparation, make sure the size of PCR products is > 500 bp. The transposase is not effective at the ends of DNA, to avoid the reduction of sequencing coverage on the ends, it is recommended to extend 50-100 bp at the both ends of the to-be-sequenced region when PCR products are prepared.

References

1. Bentley DR, Balasubramanian S, Swerdlow HP, et al. Accurate whole human genome sequencing using reversible terminator chemistry. Nature. 2008; 456(7218):53–59.

2. Wang Q, Gu L, Adey A, et al. Tagmentation-based whole-genome bisulfite sequencing. Nat Protoc. 2013; 8(10):2022–2032.

3. Buenrostro JD, Giresi PG, Zaba LC, Chang HY, Greenleaf WJ. Transposition of native chromatin for fast and sensitive epigenomic profiling of open chromatin, DNA-binding proteins and nucleosome position. Nat Methods. 2013; 10(12):1213–1218.

4. Picelli S, Faridani OR, Björklund AK, Winberg G, Sagasser S, Sandberg R. Full-length RNA-seq from single cells using Smart-seq2. Nat Protoc. 2014; 9(1):171–181.

5. Buenrostro JD, Wu B, Chang HY, Greenleaf WJ. ATAC-seq: A Method for Assaying Chromatin Accessibility Genome-Wide. Curr Protoc Mol Biol. 2015; 109:21.29.1–21.29.9.

6. Kitagawa Y, Ohkura N, Kidani Y, et al. Guidance of regulatory T cell development by Satb1-dependent super-enhancer establishment. Nat Immunol. 2017; 18(2):173–183.

7. Marie R, Pedersen JN, Bærlocher L, et al. Single-molecule DNA-mapping and whole-genome sequencing of individual cells. Proc Natl Acad Sci U S A. 2018; 115(44):11192–11197.

8. Tassetto M, Kunitomi M, Whitfield ZJ, et al. Control of RNA viruses in mosquito cells through the acquisition of vDNA and endogenous viral elements. Elife. 2019; 8:e41244.

Features

1. One tube enzymatic reaction, easy to operate

2. A single sample library process takes only 90 minutes

3. Sample input DNA volume can be as low as 1 ng, high library conversion efficiency

4. The Transposase Mix and buffers provided by the kit are subject to rigorous quality control and functional verification, so as to achieve high fidelity, stability and repeatability.

Storage

Store 5X Stop Solution at room temperature, Transposase Mix at 4°C and other components at -20°C.