Recombinant Human IL-10, Tag Free
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
The interleukin-10 (IL-10) family cytokines are powerful immune mediators with versatile functions. IL-10 family of cytokines is comprised of nine members, IL-10; IL-20 subfamily members IL-19, IL-20, IL-22, IL-24, and IL-26; and the distantly related cytokines IL-28A, IL-28B, and IL-29, which are more commonly classified as type III interferons (IFNs) and designated as IFN-λ2, IFN-λ3, and IFN-λ1[1-3].
IL-10, the founding member of this family of cytokines, was originally described as an activity purified from activated CD4+ T helper (Th) 2 cells named cytokine synthesis inhibitory factor (CSIF). Following its cloning, IL-10 was additionally found to stimulate mast cells, thymocytes, and B cells and have major immunosuppressive effects on myeloid cells[1,5-7]. IL-10 elicits its major suppressive effects on myeloid cells by inhibition of proinflammatory cytokines and antigen-presenting cells (APCs) and other functions[8,9]. IL-10 also has a direct inhibitory effect on memory Th17 and Th2 cells while promoting the survival and action of Foxp3+ regulatory T cells (Tregs) [10-13]. Compromised signaling of the IL-10 pathway is associated with inflammatory diseases such as inflammatory bowel disease (IBD) and is often accompanied by immunopathology during infections.
1. Interleukin-10 and the interleukin-10 receptor. Annu. Rev. Immunol. 2001; 19: 683-765
2. Regulation and functions of the IL-10 family of cytokines in inflammation and disease. Annu. Rev. Immunol. 2011; 29: 71-109
3. IL-10 family of cytokines. Cytokine Growth Factor Rev. 2010; 21: 315-324
4. Two types of mouse T helper cell. IV. Th2 clones secrete a factor that inhibits cytokine production by Th1 clones. J. Exp. Med. 1989; 170: 2081-2095
5. Homology of cytokine synthesis inhibitory factor (IL-10) to the Epstein-Barr virus gene BCRFI. Science. 1990; 248: 1230-1234
6. Interleukin-10. Annu. Rev. Immunol. 1993; 11: 165-190
7. Isolation and expression of human cytokine synthesis inhibitory factor cDNA clones: homology to Epstein-Barr virus open reading frame BCRFI. Proc. Natl. Acad. Sci. USA. 1991; 88: 1172-1176
8. Interleukin 10(IL-10) inhibits cytokine synthesis by human monocytes: an autoregulatory role of IL-10 produced by monocytes. J. Exp. Med. 1991; 174: 1209-1220
9. IL-10 inhibits cytokine production by activated macrophages. J. Immunol. 1991; 147: 3815-3822
10. Interleukin-10 signaling in regulatory T cells is required for suppression of Th17 cell-mediated inflammation. Immunity. 2011; 34: 566-578
11. CD4+ Th2 cells are directly regulated by IL-10 during allergic airway inflammation. Mucosal Immunol. 2017; 10: 150-161
12. Memory/effector (CD45RB(lo)) CD4 T cells are controlled directly by IL-10 and cause IL-22-dependent intestinal pathology. J. Exp. Med. 2011; 208: 1027-1040
13. Interleukin 10 acts on regulatory T cells to maintain expression of the transcription factor Foxp3 and suppressive function in mice with colitis. Nat. Immunol. 2009; 10: 1178-1184
14. Interleukin-10-deficient mice develop chronic enterocolitis. Cell. 1993; 75: 263-274
|Synonym||Interleukin-10, IL-10, Cytokine synthesis inhibitory factor, CSIF, IL10, RP11-262N9.1, IL10A, MGC126450, MGC126451, TGIF|
|Bio Activity||Determined by the dose-dependent stimulation of murine CTLL-2 cells:
ED50：< 0.1 ng/mL
Specific activity: > 1x107units/mg.
Quality Control & MSDS
- View current batch:
Purity ≥ 95%, by SDS-PAGE and HPLC.
Endotoxin Level: < 0.1 ng/μg
Components and Storage
|Formulation||The protein is dissolved in PBS buffer.|
This product is stable after storage at:
4°C for 1 week;
-20°C for 3 months.
Please avoid repeated freeze-thaw cycles.