Recombinant Human Interferon-alpha1b/IFN-α1b, Tag Free
Catalog No.
P3151
Recombinant Human Interferon-alpha1b/IFN-α1b
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IFN-αs are proteins secreted by white blood cells. They are mainly involved in the innate immune response to viral infections. There are 13 subtypes and 23 different variants of the IFN-α family. These individual proteins have molecular weights between 19-26 kDa and are composed of proteins with a length of 156-166 and 172 amino acids. All IFN-α isoforms share a common conserved sequence region between amino acid positions 115-151, while the amino terminus is variable. Many IFN-α isoforms differ in sequence only in one or two positions. Naturally occurring variants also include proteins that have 10 amino acids truncated at the carboxy terminus.
| Gene ID | 3439 |
| Accession # | P01562 |
| Alternate Names | rhIFN-α1b; IFN-α1b |
| Source | Escherichia coli. |
| Protein sequence | MCDLPETHSL DNRRTLMLLA QMSRISPSSC LMDRHDFGFP QEEFDGNQFQ KAPAISVLHE LIQQIFNLFT TKDSSAAWDE DLLDKFCTEL YQQLNDLEAC VMQEERVGET PLMNVDSILA VKKYFRRITL YLTEKKYSPC AWEVVRAEIM RSLSLSTNLQ ERLRRKE |
| M.Wt | The protein has a calculated MW of 19.5 KDa |
| Appearance | Solution protein. |
| Stability & Storage | Avoid repeated freeze-thaw cycles. It is recommended that the protein be aliquoted for optimal storage. 3 years from date of receipt, -20 to -70 °C as supplied. |
| Concentration | 1 mg/mL |
| Formulation | Supplied as a 0.2 μm filtered solution in PBS, pH7.4. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. This solution can be diluted into other aqueous buffers. |
| Biological Activity | Fully biologically active as determined by a luciferase reporter gene assay using the ISRE-Luc/HEK293 stable cell line, with an EC50 of 0.8 ng/mL. |
| Shipping Condition | Shipping with dry ice. |
| Usage | For Research Use Only! Not to be used in humans. |
Quality Control & DataSheet
- View current batch:
-
Purity > 95 % by SDS-PAGE
- Datasheet
Endotoxin: Less than 1.0 EU/μg as determined by LAL method.