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EZ Cap™ Reagent GG (3' OMe)

In stock
Catalog No.
B8179
Used for co-transcriptional capping of mRNA to form Cap 1 structure, m7(3'OMeG)(5')ppp(5')(2'OMeG)pG
Grouped product items
SizePriceStock Qty
10ul (100 mM)
$274.00
Ship with 14 days
50ul (100 mM)
$1,202.00
Ship with 14 days
100ul (100 mM)
$2,352.00
Ship with 14 days

Tel: +1-832-696-8203

Email: [email protected]

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Background

EZ Cap Reagent GG (3' OMe) is a co-transcriptional capping reagent for in vitro transcription of 5’ capped mRNA to form a Cap 1 structure.

EZ Cap GG (3' OMe) contains the 5' N7-Methyl-3'-O-Methylguanosine commonly found in mRNA capped by ARCA, whereas EZ Cap GG has a naturally occurring 5' N7-Methylguanosine.

EZ Cap GG (3' OMe) requires a GG initiator, whereas EZ Cap GG (3' OMe) typically provides >90% capped material obtaining a naturally occurring Cap 1 structure. Unlike EZ Cap AG, using EZ Cap GG (3' OMe) is possible to produce transcriptional stuttering and yield in comparable with ARCA.

EZ Cap is compatible with wild-type T7 polymerase. 5 mM EZ Cap and rNTPs is recommended.

Template design is an integral part of any transcription. EZ Cap GG (3' OMe) should be used with a specific initiating sequence 5’ GG 3’.

Final capping efficiency is dependent on the EZ Cap Reagent, DNA template and final mRNA sequence. Secondary structure due to RNA length and base composition could affect final capping efficiency.

Chemical Properties

StorageStore at -20°C or below
M.Wt1175.6 g/mol (free acid)
FormulaC33H45N15O25P4 (free acid)
SynonymsEZ Cap™ Reagent GG (3' OMe) for co-transcriptional capping of mRNA, m7(3'OMeG)(5')ppp(5')(2'OMeG)pG
SDFDownload SDF
Canonical SMILESO=C1NC(N)=NC2=C1N=CN2C3C(O)C(O)C(COP([O-])(OC4C(OC)C(N5C(N=C(N)NC6=O)=C6N=C5)OC4COP([O-])(OP([O-])(OP([O-])(OCC7OC(N8C=[N+](C)C9=C8N=C(N)NC9=O)C(O)C7OC)=O)=O)=O)=O)O3.[NH4+].[NH4+].[NH4+]
Shipping ConditionEvaluation sample solution : ship with blue ice.All other available size: ship with RT , or blue ice upon request
General tipsFor obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.

Quality Control

Quality Control & MSDS

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Chemical structure

EZ Cap™ Reagent GG (3