EZ Cap™ Reagent GG (3' OMe)
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
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A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
EZ Cap Reagent GG (3' OMe) is a co-transcriptional capping reagent for in vitro transcription of 5’ capped mRNA to form a Cap 1 structure.
EZ Cap GG (3' OMe) contains the 5' N7-Methyl-3'-O-Methylguanosine commonly found in mRNA capped by ARCA, whereas EZ Cap GG has a naturally occurring 5' N7-Methylguanosine.
EZ Cap GG (3' OMe) requires a GG initiator, whereas EZ Cap GG (3' OMe) typically provides >90% capped material obtaining a naturally occurring Cap 1 structure. Unlike EZ Cap AG, using EZ Cap GG (3' OMe) is possible to produce transcriptional stuttering and yield in comparable with ARCA.
EZ Cap is compatible with wild-type T7 polymerase. 5 mM EZ Cap and rNTPs is recommended.
Template design is an integral part of any transcription. EZ Cap GG (3' OMe) should be used with a specific initiating sequence 5’ GG 3’.
Final capping efficiency is dependent on the EZ Cap Reagent, DNA template and final mRNA sequence. Secondary structure due to RNA length and base composition could affect final capping efficiency.
|Storage||Store at -20°C or below|
|M.Wt||1175.6 g/mol (free acid)|
|Formula||C33H45N15O25P4 (free acid)|
|Synonyms||EZ Cap™ Reagent GG (3' OMe) for co-transcriptional capping of mRNA, m7(3'OMeG)(5')ppp(5')(2'OMeG)pG|
|Shipping Condition||Evaluation sample solution : ship with blue ice.All other available size: ship with RT , or blue ice upon request|
|General tips||For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.|