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EZ Cap™ Reagent AG
Used for co-transcriptional capping of mRNA to form Cap 1 structure, m7G(5')ppp(5')(2'OMeA)pG.

Catalog No.B8176
Size Price Stock Qty
10 µl (100 mM)
$111.00
In stock
50 µl (100 mM)
$513.00
In stock
100 µl (100 mM)
$933.00
In stock

Tel: +1-832-696-8203

Email: [email protected]

Worldwide Distributors

      

Sample solution is provided at 25 µL, 10mM.

Quality Control

Quality Control & MSDS

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Chemical structure

EZ Cap™ Reagent AG

EZ Cap™ Reagent AG Dilution Calculator

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EZ Cap™ Reagent AG Molarity Calculator

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Chemical Properties

Cas No. SDF Download SDF
Synonyms CleanCap™ Reagent AG for co-transcriptional capping of mRNA,m7G(5')ppp(5')(2'OMeA)pG
Canonical SMILES O=C1NC(N)=NC2=C1N=CN2C3C(O)C(O)C(COP([O-])(OC4C(OC)C(N5C(N=CN=C6N)=C6N=C5)OC4COP([O-])(OP([O-])(OP([O-])(OCC7OC(N8C=[N+](C)C9=C8N=C(N)NC9=O)C(O)C7O)=O)=O)=O)=O)O3
Formula C32H43N15O24P4 (free acid) M.Wt 1145.6 g/mol (free acid)
Solubility Storage Store at -20°C or below
Shipping Condition Evaluation sample solution : ship with blue ice.All other available size: ship with RT , or blue ice upon request
General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.

Background

EZ Cap Reagent AG is a co-transcriptional capping reagent for in vitro transcription of 5’ capped mRNA which would result in a Cap 1 structure. Cap AG has shown to provide up to 98% capping efficiency in literatures.

EZ Cap AG requires an AG initiator. EZ Cap AG typically provides >90% capped material generating a naturally occurring Cap 1 structure.

EZ Cap is compatible with wild-type T7 polymerase. 5 mM EZ Cap and rNTPs is recommended.

Template design is an integral part of any transcription. EZ Cap reagent AG can be used with a specific initiating sequence 5’ AG 3’.

Final capping efficiency depends on the EZ Cap Reagent, DNA template and final mRNA sequence. Secondary structure due to RNA length and base composition can affect final capping efficiency.