|EZ Cap™ Reagent AG
Used for co-transcriptional capping of mRNA to form Cap 1 structure, m7G(5')ppp(5')(2'OMeA)pG.
Sample solution is provided at 25 µL, 10mM.
Publications citing ApexBio Products
|Cas No.||SDF||Download SDF|
|Synonyms||CleanCap™ Reagent AG for co-transcriptional capping of mRNA,m7G(5')ppp(5')(2'OMeA)pG|
|Formula||C32H43N15O24P4 (free acid)||M.Wt||1145.6 g/mol (free acid)|
|Solubility||Storage||Store at -20°C or below|
|Shipping Condition||Evaluation sample solution : ship with blue ice.All other available size: ship with RT , or blue ice upon request|
|General tips||For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.|
EZ Cap Reagent AG is a co-transcriptional capping reagent for in vitro transcription of 5’ capped mRNA which would result in a Cap 1 structure. Cap AG has shown to provide up to 98% capping efficiency in literatures.
EZ Cap AG requires an AG initiator. EZ Cap AG typically provides >90% capped material generating a naturally occurring Cap 1 structure.
EZ Cap is compatible with wild-type T7 polymerase. 5 mM EZ Cap and rNTPs is recommended.
Template design is an integral part of any transcription. EZ Cap reagent AG can be used with a specific initiating sequence 5’ AG 3’.
Final capping efficiency depends on the EZ Cap Reagent, DNA template and final mRNA sequence. Secondary structure due to RNA length and base composition can affect final capping efficiency.