Protein A/G, used for purification of IgG antibodies, expressed in E.Coli.
Sample solution is provided at 25 µL, 10mM.
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Quality Control & DataSheet
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Purity: ≥98%, by SDS-PAGE and HPLC.
Endotoxin Level:<0.1 ng/μg
|Gene ID:||N/A||Accession #:||N/A|
|Alternate Names:||Proteina/g, protein ag, protein a/g, proteinag, protein-ag, protein-a/g, proteinag, Protein A/G, rProtein A/G, r-protein A/G, recombinant protein A/G, IgG purification, antibody purification, Protein A, Protein G, Protein A&G.|
|Source:||Escherichia Coli.||M.Wt:||59.7 kDa|
|Appearance:||Lyophilized protein||Storage:||Store at -20°C|
|Solubility:||Reconstitution in water to a concentration of 5 mg/ml gives a clear solution..|
|Shipping Condition:||Gel pack.||Handling:||Centrifuge the vial prior to opening.|
|Usage:||For Research Use Only! Not to be used in humans.|
Protein A is a 42 kDa surface protein originally found in the cell wall of the bacterium Staphylococcus aureus. It can bind immunoglobulins. Protein G is an immunoglobulin-binding protein expressed in group C and G Streptococcal bacteria. Protein A/G is a genetically engineered protein that combines the IgG binding profiles of both Protein A and Protein G. It is a gene fusion product. Recombinant fusion protein A/G contains 6× His-tag on the N-terminus, five Ig-binding regions of protein A fusion with three Ig-binding region of protein G. Cell wall binding region, albumin binding region and other non-specific binding regions have been eliminated from the fusion protein A/G to ensure the maximum specific IgG binding. 6× His-tag on N-terminus can be used for affinity purification or for protein A/G detection using anti-His-tag antibody. Protein A/G binds to all IgG subclasses from various mammalian species, including all IgGs that bind to both Protein A and Protein G, making it the ideal choice for purification of all kinds of polyclonal or monoclonal IgG antibodies.