EZ Cap™ Reagent AG (3' OMe)
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
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EZ Cap Reagent AG (3' OMe) is a co-transcriptional capping reagent for in vitro transcription of 5’ capped mRNA resulting in a Cap 1 structure. The Cap reagent has shown to provide up to 98% capping efficiency in literature.
EZ Cap AG (3' OMe) contains the 5' N7-Methyl-3'-O-Methylguanosine similarly to mRNA capped using ARCA, which is different from EZ Cap AG with a 5' N7-Methylguanosine structure.
EZ Cap AG requires an AG initiator. EZ Cap AG could typically provide >90% capped material with a naturally occurring Cap 1 structure. EZ Cap is compatible with wild-type T7 polymerase. 5 mM EZ Cap and rNTPs is recommended. Template design is an integral part of any transcription. EZ Cap reagent AG 3’OMe should be used with a specific initiating sequence 5’ AG 3’.
Final capping efficiency is dependent on the EZ Cap Reagent, DNA template and final mRNA sequence. Secondary structure due to RNA length and base composition could affect final capping efficiency.
|Storage||Store at -20°C or below|
|M.Wt||1159.6 (free acid)|
|Formula||C33H45N15O24P4 (free acid)|
|Synonyms||EZ Cap™ Reagent AG (3' OMe) for co-transcriptional capping of mRNA, m7(3'OMeG)(5')ppp(5')(2'OMeA)pG|
|Shipping Condition||Evaluation sample solution: ship with blue ice. All other available sizes: ship with RT, or blue ice upon request.|
|General tips||For obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. Stock solution can be stored below -20°C for several months.|