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EZ Cap™ Reagent AG (3' OMe)

Catalog No.
B8178
Used for co-transcriptional capping of mRNA to form Cap 1 structure, m7(3'OMeG)(5')ppp(5')(2'OMeA)pG
Grouped product items
SizePriceStock Qty
10ul (100 mM)
$167.00
Ship with 10-15 days
50ul (100 mM)
$667.00
Ship with 10-15 days
100ul (100 mM)
$1,167.00
Ship with 10-15 days

Tel: +1-832-696-8203

Email: [email protected]

Worldwide Distributors

Background

EZ Cap Reagent AG (3' OMe) is a co-transcriptional capping reagent for in vitro transcription of 5’ capped mRNA resulting in a Cap 1 structure. The Cap reagent has shown to provide up to 98% capping efficiency in literature.

EZ Cap AG (3' OMe) contains the 5' N7-Methyl-3'-O-Methylguanosine similarly to mRNA capped using ARCA, which is different from EZ Cap AG with a 5' N7-Methylguanosine structure.

EZ Cap AG requires an AG initiator. EZ Cap AG could typically provide >90% capped material with a naturally occurring Cap 1 structure. EZ Cap is compatible with wild-type T7 polymerase. 5 mM EZ Cap and rNTPs is recommended. Template design is an integral part of any transcription. EZ Cap reagent AG 3’OMe should be used with a specific initiating sequence 5’ AG 3’.

Final capping efficiency is dependent on the EZ Cap Reagent, DNA template and final mRNA sequence. Secondary structure due to RNA length and base composition could affect final capping efficiency.

Chemical Properties

StorageStore at -20°C or below
M.Wt1159.6 g/mol (free acid)
FormulaC33H45N15O24P4 (free acid)
SynonymsEZ Cap™ Reagent AG (3' OMe) for co-transcriptional capping of mRNA, m7(3'OMeG)(5')ppp(5')(2'OMeA)pG
SDFDownload SDF
Canonical SMILESO=C1NC(N)=NC2=C1N=CN2C3C(O)C(O)C(COP([O-])(OC4C(OC)C(N5C(N=CN=C6N)=C6N=C5)OC4COP([O-])(OP([O-])(OP([O-])(OCC7OC(N8C=[N+](C)C9=C8N=C(N)NC9=O)C(O)C7OC)=O)=O)=O)=O)O3.[Na+].[Na+].[Na+]
Shipping ConditionEvaluation sample solution: ship with blue ice. All other available sizes: ship with RT, or blue ice upon request.
General tipsFor obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. Stock solution can be stored below -20°C for several months.

Quality Control

Quality Control & MSDS

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Chemical structure

EZ Cap™ Reagent AG (3