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Annexin V-Cy3 Apoptosis Kit Plus
Detects Apoptotic, Necrotic & Healthy cells within 10 min

Catalog No.K2058
Size Price Stock Qty
25 assays
$174.00
In stock
100 assays
$402.00
In stock
400 assays
$870.00
In stock

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Sample solution is provided at 25 µL, 10mM.

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Related Biological Data

Annexin V-Cy3 Apoptosis Kit Plus

Related Biological Data

Annexin V-Cy3 Apoptosis Kit Plus

Overview

Kit ComponentsCatalog NoK2058
Annexin V-Cy3
SYTOX Green Dye
Binding Buffer
Product nameAnnexin V-Cy3 Apoptosis Kit Plus
Detection methodFlow cytometry (Ex = 543 nm; Em = 570 nm)
Sample typeLive cells
Species reactivityMammalian
Applications: Detecting apoptosis in living cells by flow cytometry or fluorescence microscopy. Cy3 shows brighter red fluorescence.

Features & Properties

FeaturesSimple one-step procedure; takes only 10 minutes
Fast and convenient.
Apoptotic cells show red fluorescence, dead cells show green fluorescence and live cells show little or no fluorescence.
ShippingGel pack
Storage ConditionsStore at +4°C.
UsageFor Research Use Only! Not For Use in Humans.

Description

Soon after the apoptosis is activated, most cell types transfer the membrane phospholipid phosphatidylserine (PS) from the plasma membrane inner face to the cell surface. Detection of the cell-surface PS can be easily done by staining with a fluorescent conjugate of protein Annexin V which has a robust natural affinity for PS. The one-step staining process needs just 10 minute. This assay can be directly carried out on live cells without fixation.

 

The Annexin V-Cy3 Apoptosis Detection Kit Plus includes annexin V-Cy3, SYTOX green dye, and binding buffer. The SYTOX green dye is impermeant to live cells and apoptotic cells, but stains necrotic cells with intense green fluorescence by binding to cellular nucleic acids. Following the staining the cell population with annexin V-Cy3 and SYROX Green dye in the given binding buffer, apoptotic cells exhibit green fluorescence, dead cells exhibits a higher level of green florescence and lives cells exhibits little or no fluorescence.

 

Those cell populations can be differentiated with microscopy using FITC and rhodamine filters or by flow cytometry using the FL1 channel (Ex. 488 nm/Em. 530 nm) for SYTOX Green dye and FL2 channel for Annexin V-Cy3 (Ex. 543 nm/Em. 570 nm).