Anti-DYKDDDDK (Flag) Magnetic Beads

Flag Tag is a polypeptide composed of 8 amino acid residues (DYKDDDDK), typically existing in the form of Flag or 3×Flag. It can be fused to the 5' or 3' end of a target gene via genetic recombination technology, resulting in a target protein with a Flag tag. The Flag tag is widely used in various research areas such as protein expression, purification, identification, interaction, and functional studies due to its characteristics: it does not interact with the target protein and does not affect the protein's function; the N-terminal Flag tag can be cleaved by enterokinase to obtain a tag-free target protein; and it can be detected and purified using Flag antibodies, Anti-Flag magnetic beads, or Anti-Flag affinity gel.

Anti-DYKDDDDK (Flag) Magnetic Beads are a biological research tool specifically designed for life science research—Flag tag immunomagnetic beads. They are produced by covalently conjugating high-quality Flag monoclonal antibodies to nano-scale amino magnetic beads. The Flag antibodies are immobilized on the surface of the magnetic beads, enabling specific binding to proteins containing the Flag tag. Subsequently, target proteins can be rapidly purified and detected through magnetic separation technology.

This product can be used for applications such as immunoprecipitation (IP), co-immunoprecipitation (Co-IP), protein purification, protein expression, and functional studies. It is available in two specifications: 1 mL and 2 mL. Taking a single sample (e.g., 10⁵ cells) requiring 25 μL of magnetic beads as an example, the 1 mL specification can be used for 40 samples.

The product parameters are as follows:

Q: What is the number of uses for this product (K4207)?
A: Based on a single sample (e.g., 10^5 cells) requiring 25 μL of magnetic beads, the 1 mL specification of this product can be used 40 times.
Q: What antibody is conjugated to the magnetic beads?
A: The beads are conjugated with an anti-Flag tag mouse monoclonal antibody (MA5839), which targets the same epitope and exhibits equivalent performance to Sigma's Anti-FLAG M2 antibody.
Q: What are the characteristics of the Flag-fusion proteins that can be bound?
A: The antibody recognizes the FLAG sequence at the N-terminus, Met N-terminus, and C-terminus of fusion proteins, such as DYKDDDDK Tag-Protein, Met-DYKDDDDK Tag-Protein, and Protein-DYKDDDDK Tag.
Q: What elution methods are available? Which one is recommended?
A: Three elution methods are available:   Method 1: 3X Flag Peptide Competitive Elution - This is a non-denaturing method with high efficiency, preserving the protein's native biological activity. The recommended elution product is 3XFlag (A6001).   Method 2: Acidic Elution - This is also a non-denaturing method, but its efficiency may be lower than the competitive and denaturing methods. The eluate requires neutralization. Not recommended for acid-sensitive proteins.   Method 3: SDS-PAGE Loading Buffer Denaturing Elution - The beads are boiled in an appropriate amount of loading buffer. This method is suitable for subsequent SDS-PAGE and WB analysis.
Q: Is bead aggregation during acidic elution normal?
A: Occasional bead aggregation during acidic elution is normal and does not affect performance. Adding 0.1% of a non-ionic detergent (e.g., Triton X-100, Tween-20, or NP-40) can effectively prevent aggregation without compromising antibody binding efficiency.