Protease Inhibitor Cocktail (EDTA-Free,100X in DMSO)
Crude cell extracts typically contain numerous endogenous enzymes, such as phosphatases and proteases, that can degrade proteins. During cell or tissue protein extraction in vitro, protein synthesis is significantly inhibited while degradation is enhanced. Adding inhibitors against these enzymes can improve yields of intact proteins. Protease inhibitor cocktails are used with cell lysates or tissue extracts to enhance protein stability.
Our protease inhibitor cocktail has broad specificity and serves as a general-use protease inhibitor set, suitable for purifying histidine-tagged proteins. It contains multiple critical components, including AEBSF, Aprotinin, Bestatin, E-64, Leupeptin, Phosphoramidon, and Pepstatin A, with broad inhibition specificity against serine proteases, cysteine proteases, acidic proteases, aminopeptidases, and metalloproteases. The product is supplied as a ready-to-use solution in DMSO, and can be used in WB, Co-IP, pull-down assays, IF, IHC, and kinase assays.
- 1. Shanshan Zheng, Jiao Li, et al. "Expression of urinary exosomal miR-136-5p in diabetic kidney disease and evaluation of its clinical diagnostic value." Sci Rep. 2025 Jul 4;15:23897 PMID: 40615520
- 2. Shengjie Li, Chuancai Sun, et al. "Expression and diagnostic value evaluation of urinary exosomal miR-142-3p in diabetic nephropathy." Sci Rep. 2025 Jul 4;15:23991 PMID: 40615506
- 3. Fuqi Mei, Deyu Deng, et al. "Deubiquitination of RIPK3 by OTUB2 potentiates neuronal necroptosis after ischemic stroke." EMBO Mol Med. 2025 Apr;17(4):679-695 PMID: 40021931
- 4. Daxiu Zhang, Lifeng Yu, et al. "Anaplasma phagocytophilum AFAP targets the host nucleolus and inhibits induced apoptosis." Front Microbiol. 2025 Jan 7:15:1533640 PMID: 39839117
| Catalog No. | Product Name | Summary | Targets | CAS Number | Smiles |
| A2573 | AEBSF.HCl | Serine protease inhibitor | Proteases|Serine Protease | 30827-99-7 | C1=CC(=CC=C1CCN)S(=O)(=O)F.Cl |
| A2574 | Aprotinin | Inhibitor of bovine pancreatic trypsin | Proteases|Serine Protease | 9087-70-1 | |
| A2575 | Bestatin | Aminopeptidase inhibitor | Proteases|Aminopeptidase | 58970-76-6 | CC(C)CC(C(=O)O)NC(=O)C(C(CC1=CC=CC=C1)N)O |
| A2576 | E-64 | Cysteine protease inhibitor,irreversible | Proteases|Cathepsin | 66701-25-5 | CC(C)CC(C(=O)NCCCCN=C(N)N)NC(=O)C1C(O1)C(=O)O |
| A2570 | Leupeptin | Inhibitor of serine and cysteine proteases | Proteases|Serine Protease | 103476-89-7 | O=C(C(C([H])([H])C(C([H])([H])[H])([H])C([H])([H])[H])([H])N([H])C(C([H])([H])[H])=O)N([H])C(C(N([H])C(C([H])=O)([H])C([H])([H])C([H])([H])C([H])([H])/N=C(N([H])[H])/N([H])[H])=O)([H])C([H])([H])C(C([H])([H])[H])([H])C([H])([H])[H] |
| A2571 | Pepstatin A | Aspartic protease inhibitor | Proteases|Other Proteases | 26305-03-3 | CC(C)CC(C(CC(=O)O)O)NC(=O)C(C)NC(=O)CC(C(CC(C)C)NC(=O)C(C(C)C)NC(=O)C(C(C)C)NC(=O)CC(C)C)O |
| B4790 | Phosphoramidon Disodium Salt | Metalloprotease inhibitor | Proteases | Metalloprotease | 164204-38-0 | O=C([O-])[C@@H](NC([C@@H](NP(O[C@@H]1O[C@@H](C)[C@H](O)[C@@H](O)[C@H]1O)([O-])=O)CC(C)C)=O)CC2=CNC3=C2C=CC=C3.[Na+].[Na+] |
Stored at -20°C for 12 months. | |||||