Flag-tag Protein IP Assay Kit (Agarose Gel)
The Flag tag is a polypeptide consisting of 8 amino acid residues (DYKDDDDK), usually in the form of Flag or 3X Flag. It can be linked to the 5' or 3' end of the gene of interest through genetic recombination technology to form a target protein with a Flag tag. Flag tags are widely used in the study of protein expression, purification, identification, interaction and function because they do not interact with the target protein and do not affect the function of the target protein, the N-terminal Flag tag can be excised by enterokinase to obtain the untagged target protein, and can be detected and purified by Flag antibody, Anti-Flag gel or Anti-Flag affinity gel.
This kit contains high-quality Anti-DYKDDDDK immunogel and optimized and validated necessary reagents for immunoprecipitation, making immunoprecipitation (IP, also known as pull-down) or co-immunoprecipitation (Co-IP) experiments easier and more efficient, and widely used in immunoprecipitation, co-immunoprecipitation or purification experiments of Flag-tagged fusion proteins or their protein complexes. It can be applied to a variety of sample types, such as cell lysates, cell secretion supernatants, serum, animal ascites, and other immune antigens.
This product can be used for immunoprecipitation (IP) and co-immunoprecipitation (Co-IP). Available in 10 tests and 50 tests (based on a single dose of 20 μL of Anti-DYKDDDDK immunogel).
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Components |
10 tests |
50 tests |
Storage |
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Anti-DYKDDDDK (Flag) Agarose Gel |
200 μL |
1 mL |
4°C |
|
Cell Lysis Buffer |
5 mL |
25 mL |
4°C |
|
10X TBS |
5 mL |
30 mL |
4°C |
|
Protease Inhibitor Cocktail (100X) |
50 μL |
250 μL |
-20°C |
|
5X Protein Loading Buffer (Reducing) |
200 μL |
1 mL |
-20°C |
|
Acid Elution Buffer |
1 mL |
5 mL |
4°C |
|
Neutralization Buffer |
100 μL |
500 μL |
4°C |
|
3X Flag Peptide（25X） |
80 μL |
0.4 mL |
-20°C |
|
Shipping: Blue ice |
Shelf life: 12 months |
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