HyperChrom Anti-DYKDDDDK Agarose
HyperChrom Anti-DYKDDDDK Agarose is an affinity chromatography medium based on a highly cross-linked agarose gel. The Flag tag is a polypeptide fragment (DYKDDDDK) consisting of eight hydrophilic amino acids that is localized to the surface of the fusion protein and is therefore more readily bound to antibodies and broken down by enterokinases. This chromatography medium can be used for experiments such as purification and immunoprecipitation (IP) of fusion proteins tagged with DYKDDDDK.
HyperChrom Anti-DYKDDDDK Agarose is based on a 4% agarose gel that reduces non-specific binding of heteroproteins. It can be regenerated and reused, generally 3-10 times.
HyperChrom Anti-DYKDDDDK Agarose parameters:
|
Name |
Description |
|
Chromatography media type |
Affinity chromatography media |
|
Ligation |
Anti-DYKDDDDK |
|
Scaffolding |
4% highly cross-linked agarose |
|
Average particle size |
45-165 μm |
|
Dynamic load |
>1 mg DYKDDDDK protein/mL chromatography medium |
|
Pressure-resistant |
0.1 MPa |
|
Storage solution |
1×PBS, 0.02% NaN3 |
|
Components |
1 mL |
5 mL |
25 mL |
25 mL |
100 mL |
500 mL |
|
HyperChrom Anti-DYKDDDDK Agarose |
1 mL |
5 mL |
10 mL |
25 mL |
50 mL |
100 mL |
|
Shipping: Blue ice Shelf life: Store at 4°C for 2 years |
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