Lambda Protein Phosphatase (RNase-free)
Lambda protein phosphatase (Lambda PP) is a recombinant, Mn2?-dependent enzyme expressed from bacteriophage lambda ORF221 in E. coli. It catalyzes the removal of phosphate groups from phosphorylated serine, threonine, and tyrosine residues under manganese-supplemented reaction conditions. The enzyme activity requires Mn2? ions; typical reaction buffers contain MnCl?. Lambda PP activity is effectively inhibited by addition of EDTA or by heat treatment at 65°C for 5 min. This enzyme is widely utilized in biological research to investigate protein phosphorylation dynamics, elucidate protein structure-function relationships, verify phosphorylated protein targets, and assess phospho-specific antibodies. It is provided as a purified recombinant protein solution, supplied in RNase-free storage buffer suitable for experiments involving RNA-sensitive samples.
- 1. Mengjun Zhang, Zhuang Gu, et al. "Phosphorylation-dependent charge blocks regulate the relaxation of nuclear speckle networks." Mol Cell. 2025 May 1;85(9):1760-1774.e7.
- 2. Qiaoxi Xia, Ronghui Zhong, et al. "PRMT5-mediated methylation of METTL3 promotes cisplatin resistance in ovarian cancer by facilitating DNA repair mechanisms." Cell Rep. 2025 Apr 22;44(4):115484 PMID: 40158218
| Components | 20 KU | 100 KU | 200 KU | Storage |
| Lambda PP (RNase-free) | 0.2 mL | 1 mL | 2 mL | -80°C |
| 10X Lambda PP Reaction Buffer | 1 mL | 5 x 1 mL | 10 x 1 mL | -80°C |
| 10X MnCl2 (10mM MnCl2) | 1 mL | 5 x 1 mL | 10 x 1 mL | -80°C |
Shipping: dry ice Shelf life: 2 years | ||||
Note: It is recommended to aliquot upon first use to avoid repeated freeze-thaw cycles: Store aliquots at -20°C for a validity period of 6 months.