EZ Cap™ Human IL-4 mRNA (m1Ψ)

IL-4 (interleukin-4) is a cytokine secreted by immune cells such as activated T cells, mast cells, and basophils, and belongs to the Th2 cytokine family. IL-4 plays an important role in the immune system, and its main functions include promoting B cell proliferation and differentiation, stimulating B cells to produce IgE antibodies, and participating in allergic reactions. In addition, IL-4 can induce T cell differentiation to Th2 subtype and inhibit Th1 immune response, thereby regulating immune balance. IL-4 is also involved in the replacement activation of macrophages, promoting tissue repair and anti-inflammatory responses. Clinically, IL-4 is closely related to asthma, allergic diseases and autoimmune diseases, and has become an important target for the treatment of these diseases.

EZ Cap™ Human IL-4 mRNA (m1Ψ) is provided at a concentration of ~1 mg/ml with Cap1 structure. It expresses Human IL-4 cytokine. There are currently two ways to cap mRNA: One is co-transcription method, by adding Cap analogues into the transcription process. The other is enzymatic Capping. After transcription, Cap0 capping is performed by Vaccinia virus Capping Enzyme (VCE), GTP and S-adenosylmethionine (SAM). The Cap0 is then generated into the Cap1 through 2´-O-Methyltransferase and SAM. Cap1 Capping can also be performed by adding VCE, 2´-O-Methyltransferase, GTP and SAM in a one-step process. Cap 1 structure is more ideal for mammalian systems and possess higher transcription efficiency than Cap 0 structure. The addition of N1-Methylpseudo-UTP(m1Ψ) and poly(A) tail suppress RNA-mediated innate immune activation and increase the stability and lifetime of the mRNA in vitro and in vivo. Poly(A) tail also plays an important role in enhancing the efficiency of translation initiation.