EZ Cap™ Mouse IL-23α mRNA (m1Ψ, HA tag)

IL-23α is part of interleukin-23 (IL-23), an important cytokine that belongs to the IL-12 cytokine family. IL-23 consists of two subunits: p19 (IL-23α) and p40. Il-23α is a specific subunit of IL-23 that binds to p40 to form a functional cytokine. IL-23 regulates the immune response primarily by affecting the function of T cells and natural killer cells. It is particularly important in promoting the differentiation and maintenance of Th17 cells, which play an important role in antimicrobial defense and autoimmune diseases. Abnormal expression of IL-23 has been linked to a variety of inflammatory diseases, such as psoriasis, Crohn's disease, and multiple sclerosis. As a result, IL-23 and its subunit IL-23α have emerged as potential targets for the treatment of these diseases, and many biologics have been developed to inhibit its activity.

EZ Cap™ Mouse IL-23α mRNA (m1Ψ, HA tag) is provided at a concentration of ~1 mg/ml with Cap1 structure. It expresses Mouse IL-23α cytokine with HA label, which is convenient for the subsequent detection of protein expression. There are currently two ways to cap mRNA: One is co-transcription method, by adding Cap analogues into the transcription process. The other is enzymatic Capping. After transcription, Cap0 capping is performed by Vaccinia virus Capping Enzyme (VCE), GTP and S-adenosylmethionine (SAM). The Cap0 is then generated into the Cap1 through 2´-O-Methyltransferase and SAM. Cap1 Capping can also be performed by adding VCE, 2´-O-Methyltransferase, GTP and SAM in a one-step process. Cap 1 structure is more ideal for mammalian systems and possess higher transcription efficiency than Cap 0 structure. The addition of N1-Methylpseudo-UTP(m1Ψ) and poly(A) tail suppress RNA-mediated innate immune activation and increase the stability and lifetime of the mRNA in vitro and in vivo. Poly(A) tail also plays an important role in enhancing the efficiency of translation initiation.