EZ Cap™ Mouse IL-12 mRNA (m1Ψ, HA tag)

IL-12 (Interleukin-12), a cytokine secreted by antigen-presenting cells such as dendritic cells, macrophages and B cells, plays a key role in the immune system, enhancing the body's immune response by promoting the activation and proliferation of T cells and natural killer (NK) cells, and is essential for antiviral and anti-tumor immune responses. IL-12 is not only an important molecule for the study of immune regulatory mechanisms, but also a potential target for the development of novel immunotherapeutic strategies.

EZ Cap™ Mouse IL-12 mRNA (m1Ψ, HA tag) is provided at a concentration of ~1 mg/ml with Cap1 structure. It expresses Mouse IL-12 cytokine with HA label, which is convenient for the subsequent detection of protein expression. There are currently two ways to cap mRNA: One is co-transcription method, by adding Cap analogues into the transcription process. The other is enzymatic Capping. After transcription, Cap0 capping is performed by Vaccinia virus Capping Enzyme (VCE), GTP and S-adenosylmethionine (SAM). The Cap0 is then generated into the Cap1 through 2´-O-Methyltransferase and SAM. Cap1 Capping can also be performed by adding VCE, 2´-O-Methyltransferase, GTP and SAM in a one-step process. Cap 1 structure is more ideal for mammalian systems and possess higher transcription efficiency than Cap 0 structure. The addition of N1-Methylpseudo-UTP(m1Ψ) and poly(A) tail suppress RNA-mediated innate immune activation and increase the stability and lifetime of the mRNA in vitro and in vivo. Poly(A) tail also plays an important role in enhancing the efficiency of translation initiation.